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FISH, NHL, BCL6 3q27 Rearrangement
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Test Code
BCL63Q
CPT Codes
88275, 88271 (x2)
Preferred Specimen
3 mL bone marrow or 5 mL whole blood collected in sodium heparin (green-top) tube
Minimum Volume
1 mL bone marrow • 3 mL whole blood
Other Acceptable Specimens
Bone marrow or whole blood collected in: sodium heparin (royal blue-top) or sodium heparin lead-free (tan-top) tube • 5x5mm fresh tumor tissue collected in culture transport media, or Formalin-fixed, paraffin-embedded tissue block
Instructions
Clinical history and reason for referral are required with test order. Prior therapy and transplant history should be provided with test order.
3 mL bone marrow in transport media or sodium heparin (green-top) tube or 5 mL whole blood collected in a sodium heparin (green-top) tube, or 5x5 mm fresh tumor biopsy in transport medium, or formalin-fixed, paraffin-embedded tissue. Ship at room temperature.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
3 mL bone marrow in transport media or sodium heparin (green-top) tube or 5 mL whole blood collected in a sodium heparin (green-top) tube, or 5x5 mm fresh tumor biopsy in transport medium, or formalin-fixed, paraffin-embedded tissue. Ship at room temperature.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Transport Temperature
Room temperature
Specimen Stability
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
Methodology
Fluorescence in situ Hybridization (FISH)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 7 days
Reference Range
See Laboratory Report
Clinical Significance
Chromosomal rearrangements of the BCL6 gene located at 3q27 are found in B-cell non-Hodgkin lymphomas including diffuse large cell lymphoma (DLCL),follicular lymphoma (FL) and marginal zone B-cell lymphoma (MZBCL). DLCL is an aggressive disease that usually responds well to multiagent chemotherapy. Up to 30% of DLCL demonstrate recurrent rearrangements involving the BCL6 gene.
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153
Last Updated: November 29, 2021