Heparin Monitoring Panel

Message
If unable to process and properly store specimens within 8 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.

Test Code
74141

CPT Codes
85049, 85730

Includes
Platelet count
Activated Partial Thromboplastin Time (aPTT)

Preferred Specimen
Discard tube required (do not send or use this tube)

1 EDTA lavender top tube

1 plastic (2.7mL) completely filled Sodium Citrate (light blue top) tube(s)

Minimum Volume
1 EDTA lavender top tube

1 plastic (2.7mL) completely filled 3.2% Sodium Citrate (light blue top) tube

Other Acceptable Specimens
1 EDTA 3mL or 4mL lavender top tube (Minimum fill of 1mL) or EDTA Microtainer® lavender top

1 Frozen Capped aliquots of Sodium Citrate plasma

Instructions
Use standard venipuncture and disposal techniques.

Collect the required quantity of Sodium Citrate (light blue top) tubes.

If unable to process specimens within 8 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.

EDTA lavender top tube: Gently invert 10 times immediately at time of collection (do not spin) then maintain at room temperature or refrigerate.

Sodium Citrate (light blue top) tube(s): Process immediately (stable only 8 hours), following instructions below:

Note: We recommend aliquot tubes to be made of polypropylene plastic as this material is safe to freeze at <-70 degrees C.

1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (<5/kmm3).

2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat.
Note: If the plasma shows any hemolysis, it must be recollected.

3. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.

4. Finally, place all aliquots into a <-20°C freezer within 4 hours.
Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.

Transport Container
EDTA lavender top tube(s)

Whole blood Sodium Citrate (light blue top) tube(s)
   OR
Frozen - Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)

Transport Temperature
Room Temperature: EDTA lavender top tube Whole blood
                                  Sodium Citrate (light blue top) tube(s)
Refrigerated: EDTA lavender top tube
                       Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
Frozen: Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
              Unacceptable for EDTA lavender top tube

Specimen Stability
Room Temperature: EDTA lavender top tube <24 hours
                                  Whole blood Sodium Citrate (light blue top) tube(s) < 4 hours
Refrigerated: EDTA lavender top tube <48hours
                      Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
Frozen: Unacceptable for EDTA lavender top tube
             Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended) <14 days at <−20 degrees C
                                                                                                                                             <6 months at <-70 degrees C

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
EDTA lavender top tube: Rejected for gross hemolysis, clotted, <1mL, non-EDTA tube type, >48hours old and received frozen.

Sodium Citrate Plasma: Rejected for hemolysis, clotted, improper fill, incorrect specimen type, thawed frozen aliquots, refrigerated plasma or blood, expired test tubes, >8 hours old if not processed into separated frozen plasma vials.

Methodology
Platelet Count- Automated Cell Counter, Review of peripheral smear if criteria is met

APTT - Mechanical Clot Detection

Setup Schedule
Daily
HE

Limitations
Platelet count: Platelet clumping, if present an estimate will be given i.e. decreased, adequate or increased. A numeral result is not possible if clumping is present.

Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, OH 45439



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.