Acute Leukemia Profile (FLOW Cytometry)

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

Contact Flow Cytometry (937-208-6687 or 1-800-686-2252 option 3, option 2) as soon as the specimen is collected. Any specimen collected on Friday & or the week day prior to a Holiday must be called by 4:00 pm for appropriate arrangements to be made.

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FLOW Cytometry
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CD45, CD3, CD4, CD5, CD7, CD8, CD19, CD20, kappa, lambda, CD11b, CD13, CD14, CD15, CD33, CD117, CD34, HLA-DR, CD10, TdT and additional markers as needed.

VENOUS PERIPHERAL BLOOD: 5 mL peripheral blood Sodium Heparin (dark green top) tube and 5 mL peripheral blood K2EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the EDTA lavender top tube. (minimum 2 -3 mLs)
BONE MARROW ASPIRATE: 1 mL bone marrow aspirate Sodium Heparin (dark green top) tube, 1 unstained aspirate smear, 1 wright stained aspirate smear, and 5 mL peripheral blood EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the K2EDTA lavender top tube. (minimum 1 mL)
FLUIDS: 2 mL of fluid in Sodium Heparin (dark green top) tube to prevent clotting and keep at room temperature.
CSF: 2 - 4 mL. Keep refrigerated for transport. MUST CALL prior to collection for CSF.

VENOUS PERIPHERAL BLOOD: (minimum 2 -3 mLs)
BONE MARROW ASPIRATE: (minimum 1 mL)
FLUIDS: minimum 2 mL
CSF: minimum 2 mL

Sodium Heparin and K2EDTA: Avoid hemolysis. Gently invert 10 times.

CSF = call FLOW prior to procedure for instructions

Sodium Heparin (dark green top) and K2EDTA (lavender top) tube
CSF: Refrigerate and MUST be in the lab within the hour of collection.

VENOUS PERIPHERAL BLOOD, BONE MARROW ASPIRATES, AND FLUIDS: Room Temperature and transport without delay.
CSF: Refrigerate and on ice. Must be in the lab within the hour of collection.
 

VENOUS PERIPHERAL BLOOD, BONE MARROW ASPIRATES, AND FLUIDS:
      Room Temperature =24 hours post collection
      Refrigerated = unacceptable
      Frozen = unacceptable
CSF:
      Room Temperature = unacceptable
      Refrigerated = 1 to 2 hours post collection and on ice
      Frozen = unacceptable

VENOUS PERIPHERAL BLOOD & BONE MARROW ASPIRATE: clotted, hemolyzed or greater than 24 hours old; micvrocontainers; finger or heel sticks

FLUIDS: greater than 48 hours old

CSF: greater than 4 hours old or room temperature for greater than 1 hour

Analysis performed Monday - Friday 7:00am - 6:00pm and on weekends for life threatening Acute Leukemias.
RFMVH

1 - 4 days: critical cases verbal communication with physcian.

To aid in the diagnosis, lineage assignment, subclassification, and response to treatment of hematologic malignancies.