Acute Leukemia Profile (FLOW Cytometry)

Message
Contact Flow Cytometry (937-208-6687 or 1-800-686-2252 option 3, option 2) as soon as the specimen is collected. Any specimen collected on Friday & or the week day prior to a Holiday must be called by 4:00 pm for appropriate arrangements to be made.

Test Code
71152

Alias/See Also
71152

CPT Codes
88184 x1(first marker), 88185 x# of markers minus the first marker, 88187 or 88188 or 88189 dependent upon the number of markers interpreted.

Includes
CD45, CD3, CD4, CD5, CD7, CD8, CD19, CD20, kappa, lambda, CD11b, CD13, CD14, CD15, CD33, CD117, CD34, HLA-DR, CD10, TdT and additional markers as needed.

Preferred Specimen
PERIPHERAL BLOOD: 5 mL peripheral blood Sodium Heparin (dark green top) tube and 5 mL peripheral blood EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the EDTA lavender top tube. (minimum 2 -3 mLs)
BONE MARROW ASPIRATE: 1 mL bone marrow aspirate Sodium Heparin (dark green top) tube, 1 unstained aspirate smear, 1 wright stained aspirate smear, and 5 mL peripheral blood EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the EDTA lavender top tube. (minimum 1 mL)
FLUIDS: 2 mL of fluid in Sodium Heparin (dark green top) tube to prevent clotting and keep at room temperature.
CSF: 2 - 4 mL with 1 mL of RPMI or Media. Keep refrigerated for transport. MUST CALL prior to collection for CSF.

Instructions
Sodium Heparin and EDTA: Avoid hemolysis. Gently invert 10 times.
CSF = Place 1 mL of RPMI or Media into CSF

Transport Container
Sodium Heparin (dark green top) and EDTA (lavender top) tube
CSF: Refrigerate and MUST be in the lab within the hour of collection.

Transport Temperature
PERIPHERAL BLOOD, BONE MARROW ASPIRATES, AND FLUIDS: Room Temperature and transport without delay.
CSF: Refrigerate and on ice. Must be in the lab within the hour of collection.
     For specimen integrity in the lock box click here

Specimen Stability
PERIPHERAL BLOOD, BONE MARROW ASPIRATES, AND FLUIDS:
      Room Temperature =24 hours post collection
      Refrigerated = unacceptable
      Frozen = unacceptable
CSF:
      Room Temperature = unacceptable
      Refrigerated = 1 to 2 hours post collection and on ice
      Frozen = unacceptable

Methodology
Flow Cytometry

Setup Schedule
Analysis performed Monday - Friday 7:00am - 6:00pm and on weekends for life threatening Acute Leukemias.
RFMVH

Report Available
1 - 4 days: critical cases verbal communication with physcian.

Clinical Significance
To aid in the diagnosis, lineage assignment, subclassification, and response to treatment of hematologic malignancies.


Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, OH 45439



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.