Epidermal Antibodies with Reflex to Titers

Test Code

CPT Codes
86255 (x2)

Intercellular and Basement Antibody Screens with Reflex to Titers

If Intercellular Substance Antibody Screen is positive, then Intercellular Substance Antibody Titer will be performed at an additional charge (CPT code(s): 86256).
If Basement Membrane Zone Antibody Screen is positive, then Basement Membrane Zone Titer will be performed at an additional charge (CPT code(s): 86256).

Preferred Specimen
0.5 mL serum

Minimum Volume
0.1 mL

Transport Container
Plastic screw-cap vial

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 4 days
Refrigerated: 7 days
Frozen -20° C: 30 days
Frozen -70° C: Indefinitely

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Grossly icteric

Immunofluorescence Assay (IFA)

Setup Schedule
Set up: Wed, Fri a.m.; Report available: 4-9 days

Reference Range
Intercellular Substance Ab Negative
  Titer <1:10
Basement Membrane Zone Ab Negative
  Titer <1:10

Clinical Significance
The detection of anti-skin antibodies aids in the diagnosis of chronic vesicular-bullous diseases including pemphigus, pemphigoid, cicatricial pemphigoid, and epidermolysis bullosa acquisita (EBA).
Epithelial intercellular antibodies are diagnostic for pemphigus and occur in over 90% of sera from all active forms. Antibodies to basement membrane antigens of stratified squamous epithelium occur in about 70% of active bullous pemphigoid, 50% of vesicular pemphigoid and EBA and 10% of cicatricial pemphigoid patients.

Performing Laboratory
Quest Diagnostics Nichols Inst San Juan Capistrano
33608 Ortega Highway
San Juan Capistrano, CA 92690-6130

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.