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Epidermal Antibodies with Reflex to Titers [37097]
MessageFor Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.
Test Code
AEPIAB
Alias/See Also
37097
CPT Codes
86255 (x2)
Includes
Intercellular and Basement Antibody Screens with Reflex to Titers
If Intercellular Substance Antibody Screen is positive, then Intercellular Substance Antibody Titer will be performed at an additional charge (CPT code(s): 86256).
If Basement Membrane Zone Antibody Screen is positive, then Basement Membrane Zone Titer will be performed at an additional charge (CPT code(s): 86256).
If Intercellular Substance Antibody Screen is positive, then Intercellular Substance Antibody Titer will be performed at an additional charge (CPT code(s): 86256).
If Basement Membrane Zone Antibody Screen is positive, then Basement Membrane Zone Titer will be performed at an additional charge (CPT code(s): 86256).
Preferred Specimen
0.5 mL serum
Minimum Volume
0.1 mL
Transport Container
Plastic screw-cap vial
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 4 days
Refrigerated: 7 days
Frozen -20° C: 30 days
Frozen -70° C: Indefinitely
Refrigerated: 7 days
Frozen -20° C: 30 days
Frozen -70° C: Indefinitely
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Grossly icteric
Methodology
Immunofluorescence Assay (IFA)
Setup Schedule
Set up: Wed, Fri a.m.; Report available: 4-9 days
Reference Range
| Intercellular Substance Ab | Negative |
| Titer | <1:10 |
| Basement Membrane Zone Ab | Negative |
| Titer | <1:10 |
Clinical Significance
The detection of anti-skin antibodies aids in the diagnosis of chronic vesicular-bullous diseases including pemphigus, pemphigoid, cicatricial pemphigoid, and epidermolysis bullosa acquisita (EBA).
Epithelial intercellular antibodies are diagnostic for pemphigus and occur in over 90% of sera from all active forms. Antibodies to basement membrane antigens of stratified squamous epithelium occur in about 70% of active bullous pemphigoid, 50% of vesicular pemphigoid and EBA and 10% of cicatricial pemphigoid patients.
Epithelial intercellular antibodies are diagnostic for pemphigus and occur in over 90% of sera from all active forms. Antibodies to basement membrane antigens of stratified squamous epithelium occur in about 70% of active bullous pemphigoid, 50% of vesicular pemphigoid and EBA and 10% of cicatricial pemphigoid patients.
Performing Laboratory
| Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |
Last Updated: November 29, 2021
