Desmoglein Antibodies (1 and 3) [16033X]

Test Code

DESMAB

Alias/See Also

16033

CPT Codes
83520 (x2)

Preferred Specimen

0.3 mL serum

Minimum Volume

0.1 mL

Instructions

Aseptically collect 5 to 8 mL of blood by approved venipuncture procedures.
Allow the blood to clot at room temperature (20 to 25° C) and separate as soon as possible to limit hemolysis. No anticoagulants, or preservatives should be added. Avoid using sera exhibiting a high level of lipemia, hemolysis, icterus or microbial growth. These conditions may cause aberrant test results. Do not use heat-inactivated serum as this affects test results.

Transport Container

Plastic screw-cap vial

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 4 days
Refrigerated: 7 days
Frozen: 28 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

High levels of hemolysis • High levels of lipemia • Icterus or microbial growth

Methodology
Enzyme Linked Immunosorbent Immunoassay (ELISA)

Setup Schedule

Set up: Wed, Fri; Report available: 2-7 days

Reference Range

Desmoglein 1 Antibody

Negative	<14 U/mL
Equivocal	14-20 U/mL
Positive	>20 U/mL

Desmoglein 3 Antibody

Negative	<9 U/mL
Equivocal	9-20 U/mL
Positive	>20 U/mL

Clinical Significance

To detect the presence of auto antibody specific to Desmoglein 1 and/or 3 in a patients serum as an aid to diagnose type of pemphigus

Performing Laboratory

Quest Diagnostics Nichols Inst San Juan Capistrano

33608 Ortega Highway

San Juan Capistrano, CA 92690-6130

Last Updated: June 12, 2020

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.