Hepatitis B Virus Drug Resistance, Genotype, and BCP/Precore Mutations

Test Code
10529

CPT Codes
87912

Preferred Specimen
1 mL plasma collected in a PPT potassium EDTA (white-top) tube

Minimum Volume
0.3 mL

Other Acceptable Specimens
Serum • Plasma collected in an EDTA (lavender-top) tube

Instructions
This test can only be performed reliably on specimens with a viral load of at least 600 IU/mL. For unknown viral loads order test code 16694, HBV DNA, Quantitative PCR w/Reflex to HBV Genotype.

Serum: Collect blood in sterile tubes with no anticoagulants; plastic serum separator tubes (SSTs) are recommended. Allow blood to clot at room temperature and separate serum from cells within 2 hours of collection. Transfer serum to sterile, plastic screw-capped, aliquot tubes and store refrigerated or frozen. Ship frozen.

Plasma:
Collect blood in sterile tubes containing EDTA anticoagulant; either 0.15% solution v/v final EDTA K3 (standard EDTA tube) or 9 mg spray-dried EDTA K2 (plasma preparation tube or PPT tube with plasma separator-gel, preferred). Store whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-capped aliquot tubes and store at -18° C or colder. If blood is collected in a PPT tube, centrifuge preferably within 2 hours of collection as before but it is not necessary to transfer plasma to aliquot tubes. Following centrifugation, a gel barrier maintains separation of plasma from cellular components during specimen transport and storage. The PPT is plastic and hence plasma can be stored and shipped frozen in the original tube. Avoid repeated freezing and thawing of specimen. Ship frozen.

Transport Temperature
Frozen

Specimen Stability
Room temperature: 72 hours
Refrigerated: 7 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unspun PPT tube

Methodology
Polymerase Chain Reaction (PCR) • Sequencing

Setup Schedule
Set up: Mon, Thurs evening shift; Report available: 8 days

Limitations
Results obtained from immunosuppressed patients should be interpreted with caution.

Reference Range
See Laboratory Report

Clinical Significance
This test is used to:
1. Identify HBV genotype (A-H) for epidemiology or prognostic purposes.
2. Detect hepatitis B virus (HBV) mutations associated with resistance to antiviral agents.

Performing Laboratory
Quest Diagnostics Infectious Disease, Inc.
33608 Ortega Highway, Bldg B-West Wing
San Juan Capistrano, CA 92675-2042




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.