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HIV-1 Coreceptor Tropism, Proviral DNA [91299]
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Test Code
HIV1CO
Alias/See Also
91299
CPT Codes
87906
Includes
CXCR4(X4)
Net Tropism Assessment
MVC Activity Anticipated
Net Tropism Assessment
MVC Activity Anticipated
Preferred Specimen
2 mL whole blood collected in a K2 EDTA (lavender-top) plastic tube
Minimum Volume
0.6 mL
Other Acceptable Specimens
3 mL peripheral blood mononuclear cells
Instructions
Whole blood: Collect blood in a K2 EDTA (lavender-top) tube.
Peripheral blood mononuclear cells: Collect blood in BD Vacutainer® CPT™ tube. After collection, store tube upright at room temperature for up to two hours before centrifugation. Remix blood sample immediately prior to centrifugation by inverting 10 times. Centrifuge at 800g for 30 min, room temperature with brake off. Immediately remove plasma (≥3 mL) from top layer without disturbing cell layer. Add 0.5 mL phosphate-buffered saline to remaining layer of plasma/cells. Cap tube and gently mix by inversion 10 times. Transfer the upper layer of cells (up to 3 mL) in plasma/phosphate-buffered saline to a sterile K2 EDTA tube. Store and ship frozen at <-60° C. Do not send original glass CPT™ or plasma supernatant to the lab.
Peripheral blood mononuclear cells: Collect blood in BD Vacutainer® CPT™ tube. After collection, store tube upright at room temperature for up to two hours before centrifugation. Remix blood sample immediately prior to centrifugation by inverting 10 times. Centrifuge at 800g for 30 min, room temperature with brake off. Immediately remove plasma (≥3 mL) from top layer without disturbing cell layer. Add 0.5 mL phosphate-buffered saline to remaining layer of plasma/cells. Cap tube and gently mix by inversion 10 times. Transfer the upper layer of cells (up to 3 mL) in plasma/phosphate-buffered saline to a sterile K2 EDTA tube. Store and ship frozen at <-60° C. Do not send original glass CPT™ or plasma supernatant to the lab.
Transport Container
K2 EDTA (lavender-top) plastic tube
Transport Temperature
Whole blood: Room temperature
Peripheral blood: Frozen
Peripheral blood: Frozen
Specimen Stability
Whole blood
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days
Peripheral blood mononuclear cells
Room temperature: See Collection Instructions
Refrigerated: See Collection Instructions
Frozen -20° C: See Collection Instructions
Frozen -70° C: 28 days
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days
Peripheral blood mononuclear cells
Room temperature: See Collection Instructions
Refrigerated: See Collection Instructions
Frozen -20° C: See Collection Instructions
Frozen -70° C: 28 days
Methodology
DNA Sequencing • Polymerase Chain Reaction (PCR)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Mon, Thurs; Report available: 4-8 days
Reference Range
See Laboratory Report
Clinical Significance
The use of CCR5 antagonists requires screening for viral tropism to exclude patients harboring X4 or D/M virus. HIV-1 Proviral DNA tropism test is useful for determination of viral tropism in patients with undetectable HIV-1 viral RNA (virologically suppressed).
Performing Laboratory
| Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |
Last Updated: November 30, 2021
