Y Chromosome Microdeletion, DNA Analysis

Test Code

YCHROM

Alias/See Also

14679

CPT Codes
81403<br />

Preferred Specimen

4 mL whole blood collected in an EDTA (lavender-top) tube

Minimum Volume

3 mL

Other Acceptable Specimens

ACD solution A (yellow-top) tube • ACD solution B (yellow-top) tube

Instructions

Whole blood: Specimen stability is crucial. Store and ship room temperature immediately. Do not freeze.

Extracted DNA: Please call 866-GENE-INFO (866-436-3463) for additional information.

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 8 days
Refrigerated: 8 days
Frozen: Unacceptable

Methodology
Agarose Gel Electrophoresis • Polymerase Chain Reaction (PCR)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Sets up 1 day a week.

Limitations

This assay is limited to the detection of deletions that affect the markers listed above. We are unable to determine if the absence of a single marker is caused by a deletion or a nucleotide sequence variation in the binding site for one of the PCR primers used to amplify that marker. Although rare, false positive or false negative results may occur. All results should be interpreted in the context of clinical findings, relevant history, and other laboratory data.

Reference Range

See Laboratory Report

Clinical Significance

To detect Y chromosome microdeletions associated with oligospermia and azoospermia. About 15-20% of azoospermic men and about 10% of severely oligospermic men present with microdeletions of Yq. This test targets 20 genetic loci, including those recommended by the European Quality Monitoring Network Group (Int J Andr 22:292-299 (1999).

Performing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Hwy
San Juan Capistrano, CA 92675-2042

Last Updated: June 10, 2024

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.