Platelet Antibody Screen, Serum

APAI2, APAI3

11484

GPIIb/IIIa (Cell-1)
GPIIb/IIIa (Cell-2)
GPIa/IIa (Cell-1)
GPIa/IIa (Cell-2)
GPIb/IX
GPIV
HLA Class I

1.5 mL frozen serum collected in a red-top tube (no gel)

0.5 mL

Serum should be separated from red cells prior to shipping.

Note: Erroneous results can occur from bacterial contamination of test materials, inadequate incubation periods, inadequate washing or decanting of test wells, exposure of substrate to stray light, omission of test reagents, exposure to higher or lower than prescribed temperature requirements, insufficient or excessive platelets, or omission of steps. This assay is intended for use as a screening assay. The results of this assay should not be used as the sole basis for a clinical decision. The reaction patterns of a test sample produces with this product should not be relied on solely to establish the identity of a platelet antibody. Therefore, positive or negative results obtained using this assay should be used in conjunction with clinical findings or other serological tests. Some low-titer, low-avidity antibodies may not be detected using this assay. The presence of other HPA polymorphic variants located on GPIIb/IIIa (HPA-6,7,8,9,10,11,14,16,17,19,20,21), GPIa/IIa (HPA-13,18), and GPIb/IX (HPA-12) has not been determined for the antigens captured in this kit. Antibodies to these systems may be reactive in this assay. Antibodies to low incidence HLA Class I antigens may not be detected using this product. This test has not been evaluated for the detection of autoantibodies to platelet antigens. Instead, CBPAN/Cell-Bound Platelet Autoantibody Screen, Blood should be performed.

Transport tube

Frozen

Room temperature: Unacceptable
Refrigerated: 48 hours
Frozen: 1 year

Gross hemolysis • Serum separator tube (SST®)

Mon-sat

See Laboratory Report

Platelet antibodies may be allo- or autoantibodies and may be directed to a wide range of antigenic "targets" carried on platelet cytoplasmic membranes. Serum platelet antibody test is optimized to identify the presence of platelet alloantibodies in the patient. Platelet alloantibodies are involved in several clinical situations such as:
- Immune mediated refractoriness to platelet transfusions usually due to antibodies specific to HLA class I and sometimes to antibodies specific to platelet antigens.
- Neonatal alloimmune thrombocytopenia (NAIT)
- Posttransfusion purpura (PTP), which are usually associated with platelet-specific antibodies
This test is not recommended for the diagnosis of immune thrombocytopenia (ITP) or autoimmune thrombocytopenia. Tests that are optimized to detect antibodies bound to the platelets will be useful in these situations; cell-bound platelet antibody (Direct) test is strongly recommended instead of (CBPAN/Cell Bound Platelet Auto-Antibody Screen, Blood).