| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Protein Electrophoresis, with Total Protein and Reflex to IFE, Serum
Test CodePEIFE
Quest Code
10269
CPT Codes
84155, 84165
Includes
If abnormal banding is detected, then Immunofixation will be performed at an additional charge (CPT code(s): 86334).
Preferred Specimen
4 mL serum
Patient Preparation
Overnight fasting is preferred
Minimum Volume
2 mL
Transport Container
Transport tube
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 4 days
Refrigerated: 7 days
Frozen: 28 days
Refrigerated: 7 days
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Plasma
Methodology
Capillary Zone Electrophoresis • Spectrophotometry (SP)
Reference Range
| Protein, Total, Serum | |||||
| Male (g/dL) | Female (g/dL) | ||||
| <1 Month | 4.1-6.3 | 4.2-6.2 | |||
| 1-5 Months | 4.7-6.7 | 4.4-6.6 | |||
| 6-11 Months | 5.5-7.0 | 5.6-7.9 | |||
| 1-19 Years | 6.3-8.2 | 6.3-8.2 | |||
| ≥20 Years | 6.1-8.1 | 6.1-8.1 |
| Protein Electrophoresis | |
| Albumin | 3.8-4.8 g/dL |
| Alpha-1 Globulin | 0.2-0.3 g/dL |
| Alpha-2 Globulin | 0.5-0.9 g/dL |
| Beta-1 Globulin | 0.4-0.6 g/dL |
| Beta-2 Globulin | 0.2-0.5 g/dL |
| Gamma Globulin | 0.8-1.7 g/dL |
| Abnormal Protein Band 1 | None detected |
| Abnormal Protein Band 2 | None detected |
| Abnormal Protein Band 3 | None detected |
Clinical Significance
Serum protein electrophoresis (SPE) is an analytical technique that provides separation of serum protein into six fractions: Albumin, Alpha-1, Alpha-2, Beta-1, Beta-2, and Gamma. Interpretation of elevation, decreased, or visual change in different fractions can be used as a diagnostic aid for a variety of different disease states and protein abnormalities, including monoclonal gammopathies (MG).
Performing Laboratory
| Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |
