A B C D E F G H I J K L M N O P Q R S T U V W X Y Z #

Adenovirus by PCR

Test Code
ADNVP


CPT Codes
87798

Preferred Specimen
Nasal Wash or NP Swab specimens placed in Universal Transport medium (3 mL UTM Tubes)


Instructions
  1. Nasopharynx Aspirate or Wash:　 The specimen of choice.
    1. Put a small amount of sterile, non-bacteriostatic saline into a sterile container:　
    • 0.5 - 2 ml for small children
    • 3-5 ml for adults
  3. Aspirate this into a bulb syringe for noses, or use other type of tubing/syringe combinations.
  4. Place the end of the bulb syringe or other tubing as far into the nose as comfortably possible.
  5. Squirt the saline into the nose, then aspirate the saline wash back into the bulb syringe
  6. Squirt this into a sterile container.
  7. Label container and send to lab ASAP.
  8. Lab will place into viral transprot media and immediately store at 40 C (Refrigerator).
    1. Swab:　 2 Foam tipped Dacron/polyester swabs with a plastic shaft. Wire shaft swabs are not recommended.　Obtain the proper foam-tipped swab from the stockroom.
    2. Have patient blow nose if a large amount of secretions are present prior to performing collection.
    3. Best portion is to have patient sit with head against a wall or have someone hold an infant's head.
    4. Insert Foam nasopharyngeal swab through one nostril straight back with a slight downward curve following the natural position of nasopharynx, until reaching the posterior wall of the nasopharonyx-rotate swab against the surfac of posterior nasopharynx.  NOTE:  Do not force swab-if an obstruction is encountered, try the other side.
    5. Carefully remove swab and place it in paper packet or tube of viral transport media with patient label attached.Paper packet is OK only for immediate, onsite inpatient/ED testing.  Lab will place into viral transport media.
      1. Paper packet is OK only for inpatient/ED collection sites.
      2. Viral transport media should be used if specimen is collected at remote patient sites.
    6. NOTE:  It is best to swab both nostrils by repeating the process using a fresh swab for the second side of the nasopharynx.  Plac both swabs into the same viral transport media.


Specimen Stability
Use a Nylon or Rayon tipped nasopharyngeal swab (NPS) for specimen collection.  
  • Place swab into a vial containing viral transport medium ("VTM": e.g. M4, M4-RT, M5, M6; Universal Transport Media; and Universal Viral Transport Media).
  • Specimens should be stored according to transport media manufacturer’s specifications. Specimens used for RP Flex testing must be tested or stored at 2-8°C within 4 hours of collection, regardless of manufacturer’s specifications.
  • Specimens may be stored at 2-8° C for a total of 48 hours from time of collection before testing. 
  • Once testing is complete, the original NP swab specimen tube may be stored at ≤-70° C for storage up to 30 days. One freeze/thaw is permitted if necessary for repeat testing.
  • Note: Repeat tests should be performed from original NP swab specimen.  


Methodology
PCR

Setup Schedule
24x7


Report Available
4-8 hours


Reference Range
Negative


Clinical Significance
Adenoviruses can cause a wide range of illnesses such as
  • common cold or flu-like symptoms
  • fever
  • sore throat
  • acute bronchitis (inflammation of the airways of the lungs, sometimes called a “chest cold”)
  • pneumonia (infection of the lungs)
  • pink eye (conjunctivitis)
  • acute gastroenteritis (inflammation of the stomach or intestines causing diarrhea, vomiting, nausea and stomach pain)


Performing Laboratory
CRMC Microbiology Laboratory



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.