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Viral Respiratory Panel by PCR, Viral Panel

Test Code
VRPPC


Alias/See Also
RSV, Respiratory Syncytial Virus, Parainfluenza Virus, Human Metapneumovirus, Adenovirus, Rhinovirus, influenza A Virus, Influenza B Virus


CPT Codes
87632

Includes
Includes
  • RSVPC:  RSV by PCR
  • PARIP:  Parainfluenza by PCR
  • HMPVP:  Human Metapneumovirus by PCR
  • ADNVP:  Adenovirus by PCR
  • RHINP:  Rhinovirus by PCR
  • FLUPC:  Influenza A and Influenza B  


Preferred Specimen
Nasal Wash or NP Swab specimens placed in Universal Transport medium (3 mL UTM Tubes)


Instructions
  1. Nasopharynx Aspirate or Wash:　 The specimen of choice.
    1. Put a small amount of sterile, non-bacteriostatic saline into a sterile container:　
      • 0.5 - 2 ml for small children
      • 3-5 ml for adults
    2. Aspirate this into a bulb syringe for noses, or use other type of tubing/syringe combinations.
    3. Place the end of the bulb syringe or other tubing as far into the nose as comfortably possible.
    4. Squirt the saline into the nose, then aspirate the saline wash back into the bulb syringe
    5. Squirt this into a sterile container.
    6. Label container and send to lab ASAP.
    7. Lab will place into viral transprot media and immediately store at 40 C (Refrigerator).
  2. Swab:　 2 Foam tipped Dacron/polyester swabs with a plastic shaft. Wire shaft swabs are not recommended.　Obtain the proper foam-tipped swab from the stockroom.
    1. Have patient blow nose if a large amount of secretions are present prior to performing collection.
    2. Best portion is to have patient sit with head against a wall or have someone hold an infant's head.
    3. Insert Foam nasopharyngeal swab through one nostril straight back with a slight downward curve following the natural position of nasopharynx, until reaching the posterior wall of the nasopharynx. Rotate swab against the surfac of posterior nasopharynx.  NOTE:  Do not force swab. If an obstruction is encountered, try the other nostril.

    4. Carefully remove swab and place in viral transport media with patient label attached

      1. CRMC ED: The NP swab can be placed in the paper packet and sent directly to lab. Lab will place swab into viral transport media.


Specimen Stability
Use a Nylon or Rayon tipped nasopharyngeal swab (NPS) for specimen collection.  
  • Place swab into a vial containing viral transport medium ("VTM": e.g. M4, M4-RT, M5, M6; Universal Transport Media; and Universal Viral Transport Media).
  • Specimens should be stored according to transport media manufacturer’s specifications. Specimens used for RP Flex testing must be tested or stored at 2-8°C within 4 hours of collection, regardless of manufacturer’s specifications.
  • Specimens may be stored at 2-8° C for a total of 48 hours from time of collection before testing. 
  • Once testing is complete, the original NP swab specimen tube may be stored at ≤-70° C for storage up to 30 days. One freeze/thaw is permitted if necessary for repeat testing.
  • Note: Repeat tests should be performed from original NP swab specimen.  


Methodology
PCR

Setup Schedule
24x7


Report Available
4-8 hours


Reference Range
  • RSVPC:  Negative
  • PARIP:  Negative
  • HMPVP:  Negative
  • ADNVP:  Negative
  • RHINP:  Negative
  • FLAPC:  Negative
  • FLBPC:  Negative


Clinical Significance
Refer to individual virus information


Performing Laboratory
CRMC Microbiology Laboratory



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.