Culture, Respiratory

Order Cystic for Respiratory specimens from Cystic Fibrosis patients
https://jdos.nicholsinstitute.com/dos/cmclab/test/372598/?k=CYSTIC

CXRES

Sputum Culture, Repiratory Culture, Upper Respiratory, Lower Respiratory

Additional tests and charges may apply for Identification and susceptibility, if applicable.

• Gram Stain on all lower respiratory  tract specimens.
• Organism identification
• Antibiotic Sensitivities as applicable

Sputum-5-10 ml in sterile container

Upper Respiratory Tract specimens:  Mouth, pharynx, nose, epiglottis, ear, and eye
Lower Respiratory Tract Specimens:  Sputum, ET Aspirate, Bronchial washings, Bronchial or tracheal suction material, Trans-tracheal aspirates, lung aspirates, lung biopsies.

Follow this procedure if the physician has requested an outpatient specimen collection for AFB culture or sputum culture (or other sputum specimens):
ENGLISH:  Collection of Sputum Specimen:
Collect specimen when patient first gets up in the morning, before eating:

1. Brush teeth with a wet toothbrush without toothpaste.
2. Rinse mouth and gargle with water.
3. Cough deeply from the lower chest and collect 5-10 ml sputum into the sterile container provided.
   • The testing your physician is requesting requires you to collect the specimen from a deep cough. Do not collect saliva (spit) from the mouth or postnasal discharge into the container.
4. Screw the lid carefully onto the container, and place the container inside the “zip-lock-type” bag which the lab provided. The bag has the word “Biohazard” on the outside of the bag.
5. Bring specimen to the lab within 1-2 hours. If there is a delay of more than 3 hours, refrigerate the specimen. Specimen must be delivered to the lab before 2:00 pm on the day it is collected.
6. AFB specimens are not collected in the lab facility. These sputum specimens must be collected by the patient and delivered to the lab in a sterile container.
7. Sputum specimens for pneumonia could be collected by the lab if necessary. Wear a protective mask in addition to usual protective equipment if you are assisting the patient.

 
INSTRUCCIONES PARA LA COLECTA DE FLEMA
Colecte la muestra de flema en la manana ontes de comer y ontes de lavurse la boca:

1. Cepillese los dientes, sin pasta dental.
2. Enjuagese la boca y hugu gorgusus con agua.
3. Tosu profundumente, desde la purte buja del pecho y colecte de 5-10 ml de flema en el recipiente que se le dio.
4. Cierre el recipiente, y pongulo udentro de la bolsu de plastico que se le dio en el laboratorio.
5. Traigu la muestra al laboratorio en no mus de (2) dos horus despues de la colecta, si no puede traerla en 2 horas, montengo la muestra refrigerada, la muestra tiene que estor en el laboratorio untes de las (2) dos de la torde en el dia que fue colectada.
6. La colecta de AFB no se hucen en el laboratorio, esta colecta de flema deve de ser colectada por el paciente y llevedu al laboratorio en un recipiente esterilizudo.
7. La colectu de lu flemu para pneumonia puede ser colectadu por el laboratorio, si es que es necesario, use un a muscara protectora, a purte del equipo de proteccion, si usted esti assistiendo el puciente.

NEEG KUAJ MOB DIAM NTAWV QHIA TXOG TXOJ KEV NQUS HNOOS QEEV
Thov ua raws li cov lus hauv qab no yog koj tus kws kho mob tau hais kom koj nqa hnoos qeev tuaj rau peb kuaj, xws li kaj kab mob ntsws qhauv (AFB) los yog mob rau lwm yam uas yuav siv noos qeev los mus kuaj:
Koj yuav tsum nqus thiab nti cov hnoos qeev no rau thaum sawv ntxov ua ntev koj yuav noj tshais:

1. Muab koj tus txhuam hniav tub dej los txhuam koj cov hniav – tsis txhob siv tshuaj txhuam hniav.
2. Muab dej los yaug koj lub qhov ncauj kom huv.
3. Sib zog nqus hauv kaus siab tuaj thiab hus cov hnoos qeev rau hauv lub khob uas peb muab rau koj. Koj nti kom tau ib los ob thooj loj li lub nyiaj hob rau hauv.
   • Ceebtom: Yam ua koj tus kws kho mob tau hais kom peb kuaj no yuav siv cov hnoos qeev uas nqus tob tob los xwb. Thov tsis txhob nti uab ncaug los sis tshuab ntswg rau hauv lub khob no.
4. Muab lub hau los kaw zoo zoo rau. Tas li ntawv, muab ntim rau hauv lub hnab yas uas peb muab rau koj thiab muaj lub hnab no zawm ncauj zoo zoo rau. Lub hnab no muaj npe ntawv “Biohazard” sau rau sab nrauv.
5. Thaum koj ua li no tas lawm, nqa tuaj rau tom tsev kuaj mob ua ntej ob xuab hmoos.
   Yog hais tias koj nqa tuaj tsis tau ua ntej ob xuab hmoos, koj yuav tsum muab lub hnab no cia rau hauv tub yees. Ua li cas los koj yuav tsum nqa qhov hnoos qeev no tuaj rau peb ua ntej ob hmoos tav su, tib hnub uas koj nquas tau.
6. Yog hais tias koj tuaj kuaj rau yam kab mob ntsws qhuav (AFB), peb yuav tsis pub koj nqus thiab nti rau hauv peb chav tsev kuaj mob no. Yam no yuav tsum coj mus tom tsev, thaum nqus tau lawm koj mam li nqa rov tuaj.
7. Yog hais tias koj tuaj kuaj rau yam mob ntsws paug (pneumonia) koj tuaj rau ntawv peb chav no peb mam li pab koj los tau thiab. Tab sis tus tib neeg uas pab koj yuav tsum looj lub kwb qhov ntswg thiab hnav khaub ncaws kom yuav tiv thaiv tau nws thiab nws yuav tsum looj hnab looj tes tib si.

• Transport to laboratory as soon as possible (<3 hours) at room temperature
• If > 3 hours,
  • Sputum and ET aspirates:  refrigerate at 2-8 ⁰ C.
  • Other specimens:  do not refrigerate, keep at room temperature.

24x7

2-3 days

Normal Flora

CALL all positive ET aspirates from NICU patients.

 

Cultures of specimens from the lower respiratory tract may be useful in the diagnosis of pneumonia. The lower respiratory tract is sterile; however, the upper respiratory tract is colonized with bacteria. Collecting a specimen with the least amount of upper respiratory tract contamination will provide better results for diagnosing causative organism for pneumonia.



Performed at CMC Microbiology