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Group B Strep PCR
Test CodeGRBEB
Alias/See Also
GBS, Group B, Strep B, Streptococcus B
CPT Codes
87653
Preferred Specimen
Vaginal/Rectal Swab: Cepheid GBS 2-swab Collection Device or a red swab
Instructions
Specimen is collected by the physician using the Cepheid GBS 2-swab Collection Device or a red swab.
Collect Vaginal/Rectal specimens according to the following procedure:
Collect Vaginal/Rectal specimens according to the following procedure:
- Wipe away excessive amounts of secretion or discharge.
- Remove both marked swabs from the transport container.
- Carefully insert both marked swabs into the patient's vagina. Sample secretions from the mucosa of the lower one-third part of the vagina. Rotate the swabs three times to ensure uniform sample on both swabs.
- Using the same marked swabs, carefully insert both swabs approximately 2.5 cm beyond the anal sphincter, and gently rotate to sample anal crypts.
- Place both marked swabs in the transport container.
- NOTE: If only 1 swab is received from an inpatient Labor and Delivery collection, notify the RN that the Stat testing cannot be performed. Only the culture can be performed
- Handling/Storage:
- Specimen is stable for 24 hours at room temperature.
- Specimen is stable for four (4) days at 4 degrees C (refrigerated) or up to 24 degrees C (room temperature).
- Specimen is stable for six (6) days if stored at at 2–8 degrees C.
Specimen Stability
- Specimen is stable for 24 hours at room temperature.
- Specimen is stable for four (4) days at 4 degrees C (refrigerated) or up to 24 degrees C (room temperature).
- Specimen is stable for six (6) days if stored at at 2–8 degrees C.
Methodology
Cephied Xpert GBS Assay
Setup Schedule
24x7
Reference Range
Negative
All negative intrapartum Group B Streptococcus results by PCR will be followed up with a "broth enhanced PCR."
All negative intrapartum Group B Streptococcus results by PCR will be followed up with a "broth enhanced PCR."
Clinical Significance
Group B Streptococcus (GBS) bacterial infection is associated with serious illness in newborns born to women who are colonized with the microorganism. Transmission of GBS occurs from GBS-colonized women to their newborn before birth (antepartum) or during birth (intrapartum). In the United States, GBS infection is the major cause of death in newborns who develop sepsis, pneumonia, or meningitis.
Currently, the standard of care for preventing neonatal GBS disease is screening pregnant women at 35-37 weeks of gestation to determine their GBS colonization status. Most antepartum GBS testing is performed by culture and typically takes two to three days to finalize results. This timing might be adequate for obtaining antepartum GBS culture results for the majority of women; however, some women may not have GBS results available at the onset of labor. The Cephied Xpert GBS test can be used to detect GBS directly from vaginal/rectal swab specimens in approximately 75 minutes.
CMC Laboratory Test Algorithm
Currently, the standard of care for preventing neonatal GBS disease is screening pregnant women at 35-37 weeks of gestation to determine their GBS colonization status. Most antepartum GBS testing is performed by culture and typically takes two to three days to finalize results. This timing might be adequate for obtaining antepartum GBS culture results for the majority of women; however, some women may not have GBS results available at the onset of labor. The Cephied Xpert GBS test can be used to detect GBS directly from vaginal/rectal swab specimens in approximately 75 minutes.
CMC Laboratory Test Algorithm
Performing Laboratory
CRMC Microbiology Laboratory
