A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Culture, Fungus Other source
Test CodeCXFUN
CPT Codes
87102
Preferred Specimen
- Sputum (tracheal lavage, bronchial lavage, and aerosol collection)
- Sputum should be fresh. Collect it in the early morning.
- Have patient remove dentures and rinse mouth.
- Sputum should be the result of a deep cough (not saliva) or should be induced by an aqueous aerosol.
- Collect 5 to 10 ml in sterile container.
- Respiratory specimens other than sputum: These specimens include tracheal aspirates, lung biopsy material, and bronchoscopy specimens. They are collected aseptically by physicians and immediately sent to the laboratory for examination and processing.
- Pus, exudate, and drainage: Using a sterile needle and syringe, aspirate material from undrained abscesses. Place the material in a sterile container and deliver to laboratory.
- Vaginal material: Using several sterile swabs, collect material from the vagina. Insert swabs into a sterile tube and deliver to laboratory.
- Tissue (Core biopsy, Kidney, Liver, Lymph nodes, etc)
- Collect tissue aseptically from the center and edge of the lesion.
- Place specimen between moist gauze squares, add a small amount of sterile water or 0.85% NaCl to keep tissue form drying out, and send immediately to the laboratory. Keep refrigerated for no more than 8 h at 4oC until processed.
- Bone Marrow: Aspirate approximately 3 to 5 ml of bone marrow, and place it in a sterile container. SPS or heparin can be added as an anticoagulant. The pediatric Isolator blood culture system may be used.
- CSF: Collect as much spinal fluid as possible, and place it in a sterile container. Generally, a no. 3 tube is used. Perform Cryptococcal Antigen testing. Perform gram stain on centrifuged sediment before transport to CRMC.
- Urine
- The urine specimen most suitable for making a diagnosis of mycoses of the urinary tract is a catherized specimen. Collect a clean-catch midstream specimen when aspiration or cystoscopy cannot be done.
- Collect early-morning specimens aseptically in sterile containers.
- Twenty-four-hour collections have no value. Urine may be stored at 4oC for 12 to 14 hours.
- Body fluids (pleural, synovial, and peritoneal): Collect specimens aseptically, and place them in sterile containrs.
- Hair
- No cleaning of scalp is needed.
- Select infected areas, and with forceps, epilate at least 10 hairs.
- For hairs broken off at the scalp level, use a scalpel or a blade knife.
- Place hairs in a sterile dry container for transport, or in a clean envelope abeled with the patient’s data.
- Nail
- Clean nail with 70% alcohol.
- For a specimen of the dorsal plate, scrape the outer surface and discard he scrapings. Then scrape the deeper portion for a specimen.
- Remove a portion of debris form under the nail with a scalpel.
- Collect the whole nail or nail clippings.
- Place all material in a clean envelope labeled with the patient’s data.
- Eye:
- Corneal Scrapings: Take medium to where the specimen will be collected. Physician should place corneal scrapings directly on medium.
- Intraocular Fluid: Transport intraocular fluid in a sterile tube (the collecting syringe is acceptable if handled properly and taken immediately to the laboratory), and keep the tube at 25oC (room temperature) if there is a delay in processing.
Transport Container
If processing is to be delayed for more than 4 hours, store the specimens according to the following guidelines:
- Store/Transport dermatological specimens (hair, nails, skin scrapings) in a dry container at 15-30o C (Room Temperature).
- Store/Transport blood and CSF at 30-37oC (Incubator temp)
- Store/Transport all other specimens at 4o C (Refrigerator temperature).
Setup Schedule
24x7
Reference Range
Negative
Critical Results:
Critical Results:
- CSF Specimens: Positive Cryptococcal Antigen test, positive India Ink, or gram stain showing organisms.
- Joint Fluids: Fungal elements in joint fluid specimens
- Blood Culture: Fungal elements in blood cultures.
Clinical Significance
Identification of pathogenic fungi causing infections in various body fluids or wounds.
Performing Laboratory
CRMC Microbiology