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Helicobacter pylori, IgG
Test CodeHPSEG
CPT Codes
86677
Preferred Specimen
GOLD (SST)
Other Acceptable Specimens
Red
Only Serum is acceptable
Only Serum is acceptable
Instructions
- Collect 1 gold topped, serum separator tube. No anticoagulants or preservatives should be added.
- Allow tubes to clot adequately before centrifugation.
- Avoid using hemolyzed, lipemic, or bacterially contaminated sera.
Specimen Stability
- Eight (8) hours at room temperature.
- Forty eight (48) hours at refrigerator temperature 2° and 8°C degrees.
- If delay in testing is beyond 48 hours, store test sera at -20°C or lower. Avoid multiple freeze/thaw cycles that may cause loss of antibody activity and give erroneous results.
Methodology
Elisa
Setup Schedule
M-F: 8-5
Reference Range
Index Value for H. Pylori, IgG:
The results of this test system are qualitative. The magnitude of the ratio for positive specimens may not correlate with antibody titer. If testing a particular sample occurs early during primary infection, no detectable IgG may be evident. If H. Pylori infection is suspected, a second sample should be taken at least fourteen days later.
- Negative: 0.89 IV or less: Indicates no detectable IgG antibodies to H. Pylori.
- Equivocal: 0.90 - 1.09 IV: Specimens with OD ratio values in the equivocal range should be re-tested in a few weeks or by an alternate serologic method.
- Positive: Greater 1.10 IV: Indicates a past or current infection with H. Pylori.
The results of this test system are qualitative. The magnitude of the ratio for positive specimens may not correlate with antibody titer. If testing a particular sample occurs early during primary infection, no detectable IgG may be evident. If H. Pylori infection is suspected, a second sample should be taken at least fourteen days later.
Clinical Significance
An antibody test using a blood sample is not recommended for routine diagnosis or for evaluation of treatment effectiveness. This test detects antibodies to the bacteria and will not distinguish between a present and previous infection. If the antibody test is negative, then it is unlikely that a person has had an H. pylori infection. If ordered and positive, results should be confirmed using a stool antigen or breath test.
Performing Laboratory
CRMC Microbiology/Imunology Laboratory
