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Sickle Screen.
Test CodeSICKL
Alias/See Also
Sickle, solubility test
CPT Codes
85660
Preferred Specimen
Plasma (LAV-EDTA)
Other Acceptable Specimens
Blood Product segments
Specimen Stability
Blood stored at 1-10 degrees C for up to 45 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Clotted Specimen
Methodology
Streck Sickledex test
Setup Schedule
24x7
Reference Range
Negative
Clinical Significance
Sickle cell disease is an inherited condition characterized by the presence of Hemoglobin S (Hb-S). Hb-S exists in a homozygous state (S/S) as sickle cell Anemia or in a heterozygous stayte (A/S) known as Sickle Cell Trait. Homozygous individuals (S/S) commonly exhibit symptoms of sevre hemolytic anemia and/or vascular occlusions. Heterozygous individuals (A/S) are usually asymptomatic. Hb-S may be present with other hemoglobins, such as Hemoglobin A, C or D, or thalassemia, a condition that interfers with the synthesis of normal hemoglobin.
Under conditions of low oxygen tension, the heterozygous (A/S) form can cause erythrocytes to form the characteristic sickle-shaped tacoids. The formation of these irreversibly sickled red blood cells causes the onset of the acute symptoms. Detection of both the homozygous and heterozygous condition is important so high-risk individuals can be identified and their symptoms reduced.
SICKLEDEX is a qualitative solubility test kit for testing for the presence of sickling hemoglobins in human blood or sickle control material. SICKLEDEX uses Saponin to lyse the red blood cells. Sodium Hydrosulfite then reduces the released hemoglobin. Reduced Hb-S is insoluble in the concentrated phosphate buffer and forms a cloudy, turbid suspension. Other sickling hemoglobin subtypes may also give a positive result.
Solubility tests differentiate between sickling and non-sickling hemoglobin, but do not identify the hemoglobin type. Hemoglobin types can be further differentiated by electropheresis.
Under conditions of low oxygen tension, the heterozygous (A/S) form can cause erythrocytes to form the characteristic sickle-shaped tacoids. The formation of these irreversibly sickled red blood cells causes the onset of the acute symptoms. Detection of both the homozygous and heterozygous condition is important so high-risk individuals can be identified and their symptoms reduced.
SICKLEDEX is a qualitative solubility test kit for testing for the presence of sickling hemoglobins in human blood or sickle control material. SICKLEDEX uses Saponin to lyse the red blood cells. Sodium Hydrosulfite then reduces the released hemoglobin. Reduced Hb-S is insoluble in the concentrated phosphate buffer and forms a cloudy, turbid suspension. Other sickling hemoglobin subtypes may also give a positive result.
Solubility tests differentiate between sickling and non-sickling hemoglobin, but do not identify the hemoglobin type. Hemoglobin types can be further differentiated by electropheresis.
Performing Laboratory
CRMC Laboratory
