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Complement, Total (CH50)Test Code
1 mL serum
Avoid hemolysis. Centrifuge serum specimens within 1 hour of collection. Immediately pipette serum into "sterile, plastic, screw-capped vial(s) and freeze solid" at -20°C or lower. Do not allow samples to thaw. With multiple tests, submit a separate tube for each test. Send frozen samples on dry ice to the laboratory. Do not submit the sample in a glass tube.
Plastic screw-cap vial
Room temperature: Unacceptable
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
CSF • Pleural fluid • Pericardial fluid • Synovial fluid • Received room temperature • Received refrigerated
CH50 is a screening test for total complement activity. Levels of complement may be depressed in genetic deficiency, liver disease, chronic glomerulonephritis, rheumatoid arthritis, hemolytic anemias, graft rejection, systemic lupus erythematosis, acute glomerulonephritis, subacute bacterial endocarditis and cryoglobulinemia. Elevated complement may be found in acute inflammatory conditions, leukemia, Hodgkin's Disease, sarcoma, and Behcet's Disease.
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153