Catecholamines, Fractionated, 24-Hour Urine (without Creatinine) [318X]

Test Code
318X

CPT Codes
82384

Includes
Dopamine, Epinephrine, Norepinephrine, Total Catecholamines (calculated)

Preferred Specimen
10 mL of a 24-hour urine, preserved with 25 mL 6N HCI during collection, submitted in a plastic, 24-hour urine container

Minimum Volume
5 mL

Other Acceptable Specimens
Unpreserved 24-hour urine with pH <6

Instructions
Urine with preservative should maintain a pH below 3. Urine without preservative is acceptable if pH is below 6 and the sample is shipped frozen.

Random Urine: After urine collection, add enough 6N HCL to maintain PH below 3.

Please specify on the request form and on the urine container random, timed (not 24-hour), or the total 24-hour volume.

Transport Temperature
Preserved urine: room temperature
Unpreserved urine: Frozen

Specimen Stability
Room temperature: 7 days
Refrigerated: 30 days
Frozen: 49 days

Methodology
Ultra Performance Liquid Chromatography • Electrochemical Detection • Kinetic Alkaline Picrate

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Limitations
Recent surgery, traumatic injury, upright posture, cold, anxiety, pain, clonidine withdrawal, and concurrent acute or chronic illness may produce elevated results.

Reference Range
Norepinephrine
3-8 Years 5-41 mcg/24 h
9-12 Years 5-50 mcg/24 h
13-17 Years 12-88 mcg/24 h
>17 Years 15-100 mcg/24 h
Epinephrine
3-8 Years 1-7 mcg/24 h
9-12 Years ≤8 mcg/24 h
13-17 Years ≤11 mcg/24 h
>17 Years 2-24 mcg/24 h
Dopamine
3-8 Years 80-378 mcg/24 h
9-12 Years 51-474 mcg/24 h
13-17 Years 51-645 mcg/24 h
>17 Years 52-480 mcg/24 h
Total Catecholamines
3-8 Years 9-51 mcg/24 h
9-12 Years 9-71 mcg/24 h
13-17 Years 13-90 mcg/24 h
>17 Years 26-121 mcg/24 h


Clinical Significance
The three catecholamines (norepinephrine, epinephrine, and dopamine) are the principal secretory products of neural tissue. Clinically, the measurement of circulating catecholamines is valuable in the diagnosis of catecholamine secreting tumors associated chiefly with hypertension (pheochromocytomas, neuroblastomas, and gangliomas) and with the evaluation of orthostatic hypotension.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.