Hepatitis C Viral RNA, Genotype, LiPA

Test Code

CPT Codes

Preferred Specimen
2 mL plasma collected in a PPT potassium EDTA (white-top) tube or EDTA (lavender-top) tube

Minimum Volume
0.6 mL

Other Acceptable Specimens

Separate serum or plasma from whole blood within 24 hours of collection by centrifugation at 800-1600 x g for 20 minutes at room temperature. Transfer the plasma to a properly identified, sterile, polypropylene screw-cap vial and ship refrigerated or frozen.

Transport Temperature
Refrigerated (cold packs)

Specimen Stability
Room temperature: 72 hours
Refrigerated: 14 days
Frozen: 42 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unspun PPT tube • Heparinized samples

Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)

Setup Schedule
Set up: Daily; Report available: 2-5 days

Reference Range
See Laboratory Report

Clinical Significance
Determination of hepatitis C genotype is often required to select the most appropriate direct acting agent(s) (DAA) for the treatment of hepatitis C. Some DAA's are only effective for the treatment of hepatitis C genotype 1, whereas others may be used for additional genotypes. Refer to the package inserts of the relevant DAA's for guidance. A concurrent patient HCV viral load of >=300 IU/mL is required for this test.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.