Heparin-Induced Platelet Antibody

Test Code
414

CPT Codes
86022

Preferred Specimen
1 mL random serum collected in a red-top tube (no gel)

Minimum Volume
0.5 mL

Other Acceptable Specimens
Random plasma collected in a 3.2% sodium citrate (light blue-top) tube

Instructions
Serum: Centrifuge red top tube 15 minutes at approximately 1500 g as soon as possible after clotting.
Plasma: Centrifuge blue top tube 20 minutes at approximately 2500 g. Remove plasma from the cells as soon as possible.

Transport Container
Plastic screw-cap vial

Transport Temperature
Frozen

Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 6 months

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received room temperature • Gross hemolysis • Lipemia • Icteric • Serum separator tube (SST®)

Methodology
Immunoassay (IA)

Setup Schedule
Set up: Daily; Report available: 1 day

Limitations
When heparin is stopped, antibody that may have been present disappears within weeks to months.

Reference Range
Heparin-Induced Platelet Antibody Negative
Patient O.D. ≤0.300 OD Units


Clinical Significance

Heparin can induce thrombocytopenia, a decreased platelet count, due to antibody stimulation. False positive results may be due to immune complexes. False negative results may be due to low titer, low avidity antibodies.



Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.