ANA Screen, IFA with Reflex to Titer and Pattern, IFA

Test Code

CPT Codes

If ANA Screen, IFA is positive, ANA Titer and Pattern will be performed at an additional charge (CPT code(s): 86039).

Preferred Specimen
1 mL serum

Minimum Volume
0.5 mL

Transport Container
Plastic screw-cap vial

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 4 days
Refrigerated: 7 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Microbial contamination may interfere

Immunofluorescence Assay (IFA)

Reference Range
ANA Screen Negative
ANA Titer  
<1:40 Negative
1:40-1:80 Low antibody level
>1:80 Elevated antibody level

Clinical Significance
Antinuclear antibodies are associated with rheumatic diseases including Systemic Lupus Erythematous (SLE), mixed connective tissue disease, Sjogren's syndrome, scleroderma, polymyositis, CREST syndrome, and neurologic SLE.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.