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Culture, Mycobacteria wth Fluorochrome Smear
Test CodeAFBCS
CPT Codes
87015, 87116, 87206
Includes
Acid fast stain, Mycobacterium culture, Concentration If culture is positive, identification will be performed at an additional charge (CPT code(s): 87118 or 87143 or 87149 or 87158).
Preferred Specimen
Respiratory: Sputum: Instruct the patient to gargle with water and to cough deeply to collect 5-10 mL of a respiratory specimen into a sterile leak-proof container Induced sputum: Have patient rinse mouth with water after brushing gums and tongue. With th
Transport Container
Refer to Preferred Specimen Requirements
Transport Temperature
Refrigerated (cold packs)
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Dry swab ; Expired swab transport ; Specimen in formalin or alcohol ; A bagged specimen that has leaked out and contaminated the exterior of the container ; 24-hour pooled sputum or urine ; Urine in Boricon tube
Methodology
Decontamination and concentration as needed and microscopic examination by fluorochrome stain (auramine rhodamine). Conventional and brothculture methods with identification by DNA probe, biochemical or chromatography or sequencing.
Clinical Significance
Positive smears indicate the presence of acid-fast organisms and communicability. Isolation and identification of Mycobacteria is important for the management and treatment of tuberculosis and other Mycobacterial infections. There are more than 70 species of Mycobacteria of which the primary pathogen is M. tuberculosis. Tuberculosis is a chronic, granulomatous disease caused by M. tuberculosis. Identification and if appropriate, antibiotic sensitivity is required to initiate necessary therapy.
