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Culture, Urine, Routine
Test Code395
CPT Codes
87086
Includes
If culture is positive, CPT code(s):87088 (each isolate)will beaddedwith an additional charge. Identification will be performed at an additional charge (CPT code(s): 87077or87140 or 87143 or 87147 or 87149). Antibiotic susceptibilities are only performed
Preferred Specimen
4 mL random, clean catch, midstream urine collection in gray-top urine transport tube See Urine Cultures: Routine and Special in the Bacteria section of Specimen Collection and Handling for further instructions
Instructions
Collect voided urine directly into a sterile container after cleansing the genitalia. Use syringe and needle to inject at least 4 mL into a urine culture transport tube. Urines for routine bacterial culture will only be accepted in the 4 mL culture transport tubes made by Becton-Dickinson, which we provide. An exception will be made for small volume collections of less than 4 mL which must be transported refrigerated in a sterile screw-cap container. Volumes of less than 4 mL should not be transported in the gray-top tubes.
Transport Container
Gray-top urine transport tube from urine collection kits Sterile container for specimens
Transport Temperature
Preserved: Room temperature Unpreserved: Refrigerated (cold packs)
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
If urinalysis transport tube is received ; Unpreserved urine >5 mL ; Preserved urine >2 days old ; Urinetube not filled to minimum fill line ; Foley tips ; Frozen specimens ; Specimens from catheter bag ; Urine in urinalysis transport tube
Methodology
Semi-quantitative culture techniques and conventional or rapid identification methods
Clinical Significance
This culture is designed to quantitate the growth of significant bacteria when collected by the Clean Catch Guidelines or from indwelling catheters. Quantitative culturing of urine is an established tool to differentiate significant bacteruria from contamination introduced during voiding.This test has a reference range of less than 1,000 bacteria per mL.More than 95% of Urinary Tract Infections (UTI) are attributed to a single organism. Infecting organisms are usually present at greater that 100,000 per mL, buta lower density may be clinically important. In cases of UTI where more than one organism is present, the predominant organism is usually significant and others are probably urethral or collection contaminants. When multiple organisms are isolated from patients with indwelling catheters,UTI is doubtful and colonization likely.
