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Clostridium difficile Toxin A and B, EIA
Test CodeCDIFF
CPT Codes
87324
Preferred Specimen
5 grams (5 mL) stool
Instructions
Collect fresh stool in sterile, leak-proof container without media, preservative, or metal ion. For patients requiring the use of diapers, first line the diaper with clean plastic to prevent absorption. Then transfer 5 g or 5 mL of the stool specimen from the plastic lined diaper to the sterile container. Do not submit the diaper itself. Cap securely. Do not use M4 transport media. Do not use any preservative, media or additive.
Transport Container
Clean plastic screw-cap, leak-proof container
Transport Temperature
Refrigerated (cold packs) ;Frozen
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Stools submitted in transport media or swab ; Unfrozen stools greater than 72 hours old
Methodology
Immunoassay
Clinical Significance
This test offers maximum sensitivity as it detects both toxinA and toxinB produced by C. difficile. Clostridium difficile has been associated with colitis secondary to antimicrobial therapy.
