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14-3-3 Protein, CSF (Prion Disease)
MessageThe following forms are required and must be submitted with the sample. Forms can be found in Additional Information below.
- Prion Test Request Form
- Prion Testing and Reporting Policies Form
- Prion Billing Form (if Prion Center is billing patient directly)
Test Code
PRIOD
Alias/See Also
37989
CPT Codes
0035U, 83520 (x2)
Preferred Specimen
2 mL frozen CSF collected in a sterile screw-cap vial
Minimum Volume
1 mL
Other Acceptable Specimens
Frozen CSF collected in a Sarstedt CSF false-bottom tube
Instructions
The patient must be 12 years of age or older.
Cerebrospinal Fluid (CSF) obtained by lumbar puncture. The first 2 mL of CSF that flows from the tap should be discarded. Collect 2 mL of CSF (1 mL minimum), avoiding bloody tap. The sample must be immediately frozen, at least in a -20° C freezer. Freeze within 20 minutes of collection. Store and transport frozen. Ship using a styrofoam container with sufficient dry ice (5 lbs/24 hours).
Required patient information: Please complete and send the National Prion Requisition "Test Request Form", available on the website at National Prion Disease Pathology Surveillance Center with the sample.
*** Ship Monday through Wednesday (priority overnight) ***
Cerebrospinal Fluid (CSF) obtained by lumbar puncture. The first 2 mL of CSF that flows from the tap should be discarded. Collect 2 mL of CSF (1 mL minimum), avoiding bloody tap. The sample must be immediately frozen, at least in a -20° C freezer. Freeze within 20 minutes of collection. Store and transport frozen. Ship using a styrofoam container with sufficient dry ice (5 lbs/24 hours).
Required patient information: Please complete and send the National Prion Requisition "Test Request Form", available on the website at National Prion Disease Pathology Surveillance Center with the sample.
*** Ship Monday through Wednesday (priority overnight) ***
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: Indefinitely
Refrigerated: Unacceptable
Frozen: Indefinitely
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Bloody and insufficient sample quantity • Samples that are colored or contain blood cannot be performed for RT-QuIC and 14-3-3 GAMMA
Methodology
Immunoassay • RT-QuIC
Setup Schedule
Set up: Varies; Report available: 7 days
Reference Range
See Laboratory Report
Clinical Significance
In CSF: Search for the presence of the 14-3-3 protein. The 14-3-3 protein is a marker for some prion diseases, such as Creutzfeldt-Jakob disease (CJD), when a number of other neurodegenerative conditions are excluded.
Performing Laboratory
National Prion Disease Pathology Surveillance Center
Case Western Reserve University
2085 Adelbert Road, Room 419
Cleveland, OH 44106
Additional Information
Prion Test Request Form
Prion Testing and Reporting Policies Form
Prion Billing Form
Last Updated: May 6, 2024
