5-Hydroxyindoleacetic Acid (5-HIAA), Random Urine with Creatinine

Test Code

Alias/See Also

CPT Codes
82570, 83497


Preferred Specimen
10 mL random urine with 6N HCl submitted in a plastic, screw-cap container

Patient Preparation
Patient should avoid food high in indoles: avocado, banana, tomato, plum, walnut, pineapple, and eggplant

Minimum Volume
5 mL

Other Acceptable Specimens
Random urine, unpreserved, collected in a sterile screw-cap container


After urine collection, add 6N HCl to maintain a pH below 3.

Urine without preservative is acceptable if pH is below 6 and the sample is shipped frozen.
Record patients age on test request form and urine vial.

Transport Temperature
Preserved: Room temperature
Unpreserved: Frozen

Specimen Stability
Room temperature: 7 days
Refrigerated: 30 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
pH >3.0 and received unfrozen

High Performance Liquid Chromatography (HPLC) • Electrochemical Detection

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Monday-Friday Morning Report available: 2 Days after receipt at the performing laboratory. Add three (3) days for transport.

Patients with renal disease may display falsely decreased results. Urinary 5-HIAA is increased in patients with malabsorption disorders who also display a larger concentration of urinary tryptophan metabolites. Urinary 5-HIAA is increased in patients with intestinal obstruction and with some noncarcinoid islet cell tumors. Carcinoid syndrome may not cause elevated results, especially if the patient does not have diarrhea.

Reference Range
5-HIAA, Random Urine
<2 YearsNot established
2-10 Years≤12.0 mg/g creat 
>10 Years≤10.0 mg/g creat
Creatinine, Random Urine
≤6 Months2-28 mg/dL
7-11 Months2-31 mg/dL
1-2 Years2-110 mg/dL
3-8 Years2-130 mg/dL
9-12 Years2-160 mg/dL
>12 Years Male20-320 mg/dL
>12 Years Female20-275 mg/dL

Clinical Significance
Values above 10 mg/24 hrs occur in carcinoid syndrome. Slight elevations also occur in nontropical sprue and after reserpine administration.
Drugs which can interfere with testing due to physiologic response: Acetaminophen, Alpha and Beta Blockers, Atenolol, Bromocriptine, Bronchodilators, Clonidine, Digoxin, Isoniazid, L-Dopa, Labetalol, Methyldopa, Monoamine Oxidase Inhibitors, Nitroglycerin, Sympathomimetic Amines, Phenobarbital, Phenothiazines, Phentolamine, Reserpine, Salicylates, Tricyclic Antidepressants

Performing Laboratory
Quest Diagnostics Nichols Inst San Juan Capistrano
33608 Ortega Highway
San Juan Capistrano, CA 92690-6130

Last Updated: August 4, 2023

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.