MSI for LYNCH SCREENING

Test Code

MSILN

CPT Codes
81301; 88381

Includes

This assay detects instability at genotypes ACVr2; BTBD7; DIDO1; MRE11; RYR3; SEC31 and SULF2
Includes Pathologist interpretation
Mutation Detections:
- ACVR2A- 2q22.3-q23.1
- BTBD7 - 14q32.12
- DIDO1 - 20q13.33
- MRE11 - 11q21
- RYR3 - 15q13.3-q14
- SEC31A - 4q21.22
- SULF2 - 20q13.12

Preferred Specimen

Please send 2-5 slides (>50mm tissue area) or paraffin block and 1 H&E slide with the area of invasive tumor circled. Send tissue block to Molecular in a red pathology interoffice envelope. AP request form must be included with order.

Other Acceptable Specimens

None

Transport Temperature

Room temperature

Specimen Stability

Room temperature - cut slides are stable 60 days. Paraffin blocks are stored for 10 years.

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Less than 20% tumor cells
Samples sent frozen
Decalcified specimens

Methodology
Real Time Polymerase Chain Reaction (PCR)

Setup Schedule

No set day

Report Available

Report available within 3 days of receipt at the performing laboratory.

Reference Range

Microsatellite Stable / Intact DNA mismatch repair detected (MSS/MSI-L)

Clinical Significance

Useful for Lynch Syndrome screenings and to determine potential eligibility for immunotherapy by identifying microsatellite instability-high (MSI-H) cancers.

Performed By
Alverno Laboratories

Performing Laboratory
Alverno Central Lab
NMB18 (Molecular Biology, Idylla)

Last Updated: June 8, 2023

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.