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ANTINUCLEAR ANTIBODY by IFA w/REFLEX to MULTIPLEX FLOW IMMUNOASSAY
Test CodeANARB
Alias/See Also
ANABZ
CPT Codes
86039; If reflexed, add 86235 x10 and 86225
Includes
Titer and Pattern. If test is reflexed, the quantitative detection of dsDNA, semi-quantitative detection of Chromatin, Ribosomal P, SS-A, SS-B, Sm, SmRNP, RNP, Scl-70, Jo-1, and Centromere B.
Preferred Specimen
1 Gold tube (gel separator)
Minimum Volume
0.5 mL
Other Acceptable Specimens
None
Instructions
Centrifuge for complete separation of serum and red cells. Refrigerate until tested.
Transport Temperature
Refrigerate
Specimen Stability
- 2-8°C up to 2 days; otherwise freeze
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed or lipemic samples
Methodology
IFA, if reflexed Multiplex Flow Immunoassay
Setup Schedule
M, W, F (day shift only)
Please note, if reflex IFA is warranted, it will be performed on the next testing day.
Please note, if reflex IFA is warranted, it will be performed on the next testing day.
Report Available
Fianl report may take up to 72 hours.
Reference Range
<1:40
Clinical Significance
This test panel starts with IFA where cytoplasmic and nuclear immunofluorescence patterns on Hep-2 cells are used to detect the presence of antibodies associated with systemic autoimmune diseases which may be helpful in the diagnosis of Systemic Lupus Erythematosis, Scleroderma, Mixed Connective Tissue disease, Undifferentiated Connective Tissue disease, Sjogren&rsquo s syndrome, Dermatomyositis, Polymyositis, primary biliary cirrhosis and auto immune hepatitis.
If the titer of any pattern identified is >/=1:80, Bioplex testing will be performed and all antibodies will be reported. The Bioplex method utilizes a multiplex bead assay to quantitatively detect Chromatin, Ribosomal P, SS-A 60, SS-A 52, SS-B, Sm, SmRNP, RNP A, RNP 68, Scl-70, Jo-1 and Centromere B. Proprietary software is used to report an association between the antibodies, patterns, and diseases. Positive IFA titers of 1:40 can be detected in up to 32% of healthy individuals, stressing the importance of clinical correlation with patient signs and symptoms.
If the titer of any pattern identified is >/=1:80, Bioplex testing will be performed and all antibodies will be reported. The Bioplex method utilizes a multiplex bead assay to quantitatively detect Chromatin, Ribosomal P, SS-A 60, SS-A 52, SS-B, Sm, SmRNP, RNP A, RNP 68, Scl-70, Jo-1 and Centromere B. Proprietary software is used to report an association between the antibodies, patterns, and diseases. Positive IFA titers of 1:40 can be detected in up to 32% of healthy individuals, stressing the importance of clinical correlation with patient signs and symptoms.
Performed By
Alverno Laboratories
Performing Laboratory
Alverno Central Lab
NIMEU (Immunology, Euroimmun)
Last Updated: June 8, 2023
