ANTINUCLEAR ANTIBODY QUANTITATIVE TITER by IFA

Message

This test does not include a screen; ANA by IFA only

Test Code

ANAIF

Alias/See Also

ZANQT

CPT Codes
86039

Preferred Specimen

1 Gold tube (gel separator)

Minimum Volume

1 mL

Other Acceptable Specimens

None - alternate tube types are no longer acceptable.

Instructions

Centrifuge for complete separation of serum and red cells. Refrigerate until tested.

Transport Temperature

Refrigerate

Specimen Stability

15-30°C up to 8 hours
2-8°C up to 2 days; otherwise freeze

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolyzed or lipemic samples

Methodology
IFA

Setup Schedule

M, W, F (day shift only)

Reference Range

<1:40

Clinical Significance

This test utilizes cytoplasmic and nuclear immunofluorescence patterns on HEp-2 cells to detect the presence of antibodies associated with systemic autoimmune diseases which may be helpful in the diagnosis of: systemic lupus erythematosus, scleroderma, mixed connective tissue disease, undifferentiated connective tissue disease, Sjogrens syndrome, dermatomyositis, polymyositis, primary biliary cirrhosis and auto immune hepatitis.

The test is intended for diagnostic use and is of limited clinical utility in monitoring disease course. Overlap may occur between patterns, antibodies and diseases. If a negative result is obtained in the presence of clinical signs and symptoms suggestive of systemic autoimmune disease, repeat testing using an alternative method is suggested (Multiplex bead testing/Bioplex). Positive IFA titers of 1:40 can be detected in up to 32% of healthy individuals, stressing the importance of clinical correlation. with patient signs and symptoms.

Performed By
Alverno Laboratories

Performing Laboratory
Alverno Central Lab
NIMEU (Immunology, Euroimmun)

Last Updated: April 25, 2023

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.