NEXT GEN SEQUENCING COMPREHENSIVE PANEL

Message

Tissue block should have a high tumor (>10%) concentration. Avoid necrotic areas. No decalcification or other fixative.

Order NGSCP panel and provide an AP requisition

Test Code

NGSCP

CPT Codes
81455; 88381

Includes

500+ gene multiplexed DNA and RNA next generation sequencing panel. Assay detects single nucleotide variations, copy number alterations, deletions / insertions, gene fusions, MSI and TMB.

Preferred Specimen

12 unstained 5-micron slides

Minimum Volume

Tumor concentration >10%
8 slides

Transport Temperature

Room temperature

Specimen Stability

Paraffin blocks are stable up to 10 years
5-micron sections mounted on slides up to 6 months at room temperature

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Unacceptable Conditions:

Frozen
Absence of or <10% tumor cells
Decalcified samples
Fixative other than formalin

Methodology
Next Generation Sequencing

Setup Schedule

Batched once per week

Report Available

Report available 7-14 days after set up.

Reference Range

No alterations identified within tested genes

Clinical Significance

Testing is indicated in patients that have any solid tumor AND, advanced stage (III/IV) disease, metastatic, relapsed/recurrent or refractory to therapy AND, not previously tested by same NGS test AND, seeking further treatment for tumor.

Performed By
Alverno Laboratories

Performing Laboratory
Alverno Central Lab
NMBNG (Molecular Biology, Next Gen Sequencing)

Additional Information
AP Patient Information Sheet

Last Updated: June 7, 2023

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.