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Creatine Kinase Isoenzymes (CK Isoenzymes) with Total CK
Test CodeCKIEZ
Alias/See Also
4451
CPT Codes
82550, 82552
Includes
CK-BB, CK-MB, CK-MM and Creatine Kinase (CK), Total
Preferred Specimen
2 mL frozen serum
Minimum Volume
1 mL
Instructions
Allow blood to clot at room temperature for 30 minutes. Centrifuge immediately to separate the serum from cells and pour serum into a plastic transport tube. If CK and CK isoenzymes are ordered together, specimen must be submitted frozen.
Transport Container
Transport tube
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 48 hours
Frozen: 14 days
Refrigerated: 48 hours
Frozen: 14 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Received thawed
Methodology
Electrophoresis • Enzymatic
Setup Schedule
Set up: Sun-Fri; Report available: 2-3 days after receipt at the performing laboratory. Add two (2) days for transport.
Reference Range
See Laboratory Report
Clinical Significance
This test measures creatine kinase (CK), an enzyme found primarily in striated muscle and heart tissue, and may be useful in assessing muscle damage. Total CK and fractions of CK isoenzymes are reported.
CK is a dimeric enzyme composed of either 2 B subunits (CK-BB), 2 M subunits (CK-MM), or an M and a B subunit (CK-MB). CK-MM is the primary isoenzyme found in the skeletal muscle and heart tissue. CK-BB is mainly found in the brain and smooth muscle of gastrointestinal tract and urinary bladder. CK-MB is mainly found in the heart with a small amount in skeletal muscle [1].
An increase in the CK level is often observed in inflammatory myopathy (eg, viral myositis, polymyositis, and immune-mediated myopathies), muscular dystrophy (eg, Duchenne sex-linked muscular dystrophy), rhabdomyolysis, or malignant hyperthermia [1]. In patients with neuromuscular disorders, an increased CK level may be the only initial manifestation [1]. Other causes of elevated CK levels include hypothyroidism, direct muscle trauma (eg, surgery and intramuscular injection), excessive exercise, and certain medications (eg, statins, fibrates, antiretrovirals, and angiotensin II receptor antagonists) [1].
The quantitation of CK-MB levels in serum was widely used to diagnose acute myocardial infarction but has been replaced by troponin I and T levels, which are more cardiac-specific [2,3]. CK-MB measurement, preferably expressed as CK-MB relative to the total CK level, is only indicated in patients with suspected acute coronary syndrome or reinfarction when troponin T and I testing are not available [2]. In individuals with chronic muscle damage/disease or chronic renal failure, CK-MB may account for the elevation of CK levels owing to the phenomenon of "fetal reversion" [1].
CK-BB levels may be increased in newborns with brain damage or very low birth weight, although healthy newborns can also have increased CK-BB levels as a result of birth-related muscle trauma [1].
The results of this test should be interpreted in the context of pertinent clinical and family history and physical examination findings.
References
1. Panteghini M, et al. Serum enzymes. In: Rifai N, et al. eds. Tietz Textbook of Laboratory Medicine. 7th ed. Elservier Inc; 2022:4149-4299.
2. CKMB: optimal testing recommendations. AACC. Accessed October 11, 2022. https://www.aacc.org/advocacy-and-outreach/optimal-testing-guide-to-lab-test-utilization/a-f/ckmb
3. Gulati M, et al. Circulation. 2021;144(22):e368-e454.
CK is a dimeric enzyme composed of either 2 B subunits (CK-BB), 2 M subunits (CK-MM), or an M and a B subunit (CK-MB). CK-MM is the primary isoenzyme found in the skeletal muscle and heart tissue. CK-BB is mainly found in the brain and smooth muscle of gastrointestinal tract and urinary bladder. CK-MB is mainly found in the heart with a small amount in skeletal muscle [1].
An increase in the CK level is often observed in inflammatory myopathy (eg, viral myositis, polymyositis, and immune-mediated myopathies), muscular dystrophy (eg, Duchenne sex-linked muscular dystrophy), rhabdomyolysis, or malignant hyperthermia [1]. In patients with neuromuscular disorders, an increased CK level may be the only initial manifestation [1]. Other causes of elevated CK levels include hypothyroidism, direct muscle trauma (eg, surgery and intramuscular injection), excessive exercise, and certain medications (eg, statins, fibrates, antiretrovirals, and angiotensin II receptor antagonists) [1].
The quantitation of CK-MB levels in serum was widely used to diagnose acute myocardial infarction but has been replaced by troponin I and T levels, which are more cardiac-specific [2,3]. CK-MB measurement, preferably expressed as CK-MB relative to the total CK level, is only indicated in patients with suspected acute coronary syndrome or reinfarction when troponin T and I testing are not available [2]. In individuals with chronic muscle damage/disease or chronic renal failure, CK-MB may account for the elevation of CK levels owing to the phenomenon of "fetal reversion" [1].
CK-BB levels may be increased in newborns with brain damage or very low birth weight, although healthy newborns can also have increased CK-BB levels as a result of birth-related muscle trauma [1].
The results of this test should be interpreted in the context of pertinent clinical and family history and physical examination findings.
References
1. Panteghini M, et al. Serum enzymes. In: Rifai N, et al. eds. Tietz Textbook of Laboratory Medicine. 7th ed. Elservier Inc; 2022:4149-4299.
2. CKMB: optimal testing recommendations. AACC. Accessed October 11, 2022. https://www.aacc.org/advocacy-and-outreach/optimal-testing-guide-to-lab-test-utilization/a-f/ckmb
3. Gulati M, et al. Circulation. 2021;144(22):e368-e454.
Performing Laboratory
| Quest Diagnostics Nichols Institute |
| 14225 Newbrook Drive |
| Chantilly, VA 20153 |
Last Updated: May 4, 2023
