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Direct Antiglobulin Test
MessageLabeling must be performed at the patient’s bedside, immediately following specimen collection, by the person who collected the specimen.
Specimens will be rejected if there is evidence that the labeling did not take place at the bedside (i.e. double labeling or additional labeling beneath the blood bank label).
Specimens will be rejected if there is evidence that the labeling did not take place at the bedside (i.e. double labeling or additional labeling beneath the blood bank label).
Test Code
DAT
Alias/See Also
DAT, Direct Coombs
Includes
ABO Group, Rh Type, and DAT
Preferred Specimen
10 mL whole blood (2 mL minimum)
Instructions
Must be legibly labeled with the following:
A. Patient’s complete name
B. Patient’s medical record number
C. Date and time of collection
D. Identification of person collecting the specimen
Specimens may be labeled with an LIS label, hospital generated label, or handwritten label. The patient name may be truncated due to space limitations on the label; these are acceptable.
Transport Container
EDTA (lavender/purple) preferred.
Specimen Stability
Room temperature: 24 hours
Refrigerated: <10 days
Frozen: Unacceptable
Refrigerated: <10 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Any collection container containing serum-separator (SST)
Improper, incorrect or incomplete labeling
Improper, incorrect or incomplete labeling
Report Available
Same day
Limitations
Specimen can not be used for compatibility testing (crossmatch)
