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Mycobacterium Blood culture, ID & Sensitivity
Test Code8518
Preferred Specimen
Sodium heparin is no longer an acceptable container type.
Bactec(R) Myco/F Lytic aerobic blood bottle is the only acceptable container type.
If positive for Mycobacterium, this test progresses to an identification and sensitivity on all isolates.
5 mL whole blood/bone marrow, inoculated into a Bactec(R) Myco/F Lytic aerobic blood bottle (1 mL minimum)
Bactec(R) Myco/F Lytic aerobic blood bottle is the only acceptable container type.
If positive for Mycobacterium, this test progresses to an identification and sensitivity on all isolates.
5 mL whole blood/bone marrow, inoculated into a Bactec(R) Myco/F Lytic aerobic blood bottle (1 mL minimum)
Transport Container
inoculated into a Bactec(R) Myco/F Lytic aerobic blood bottle
Transport Temperature
Room Temperature
Specimen Stability
RT: 48 hours
Refrigerated (cold packs): Unacceptable
Frozen: Unacceptable
Refrigerated (cold packs): Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Rejection Criteria:
- Received frozen
- Specimens in EDTA (lavender-top tube)
- Specimens in heparin (green-top tube)
- Specimens in citrate, ACD, or SPS (yellow-top tubes)
- Bactec(R) plus aerobic/f (silver label with gray-top) bottles
- Bactec(R) lytic/10 anaerobic/f (purple label and cap) bottles
- Bactec(R) peds bottle
Methodology
DNA Geneprobes & Biochemicals
Setup Schedule
Daily M,N
Report Available
7 weeks
Reference Range
No acid-fast bacilli isolated
