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CBC with DIFF
Test CodeCBC
Alias/See Also
Complete Blood Count, Hemogram & diff
Includes
WBC,RBC,HGB,HCT,MCV,MCH,MCHC,RDW, PLT, MPV, differential
Preferred Specimen
Adult: 5-7mL whole blood, based on tube size (1.0mL minimum)
Pediatric: 3mL whole blood (0.5mL minimum)
Neonate: 0.5mL whole blood in microtainer
Pediatric: 3mL whole blood (0.5mL minimum)
Neonate: 0.5mL whole blood in microtainer
Transport Container
EDTA (lavender/purple top)
Specimen Stability
Room temperature: 48 hours, Refrigerated: 2 days, Frozen: unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Clotted or grossly hemolyzed specimens are unacceptable.
Limitations
The following will interfere with results: Fibrin, bacterial contamination, platelet clumps, abnormal proteins, cold agglutinins, extreme temperature conditions, resistant hemoglobin, abnormal chemistries and specimens older than 48 hours.
Reference Range
ADULT CBC AND DIFFERENTIAL REFERENCE RANGES
| Parameter / Units of measurement | Male | Female |
| WBC / x10(3)/mcL | 4.2 – 9.1 | 4.0 – 10.0 |
| RBC / 106/µL | 4.63 – 6.08 | 3.93 – 5.22 |
| HGB / g/dL | 13.7 – 17.5 | 11.2 – 15.7 |
| HCT / % | 40.1 – 51.0 | 34.1 – 44.9 |
| MCV / fL | 79.0 – 92.2 | 79.4 – 94.8 |
| MCH/ pg | 25.7 – 32.2 | 25.6 – 32.2 |
| MCHC / g/dL | 32.3 – 36.5 | 32.2 – 35.5 |
| RDW / % | 11.6 – 14.4 | 11.7 – 14.4 |
| PLT / x10(3)/mcL | 163 – 337 | 182 – 369 |
| MPV / fL | 9.4 – 12.4 | 9.4 – 12.3 |
| Absolute Neutrophils / x10(3)/mcL | 1.78 – 5.38 | 1.56 – 6.13 |
| Absolute Lymphs / x10(3)/mcL | 1.32 – 3.57 | 1.18 – 3.74 |
| Absolute Monocytes / x10(3)/mcL | 0.30 – 0.82 | 0.24 – 0.86 |
| Absolute Eosinophils / x10(3)/mcL | 0.04 – 0.54 | 0.04 – 0.36 |
| Absolute Basophils/ x10(3)/mcL | 0.01 – 0.08 | 0.01 – 0.08 |
| Nucleated RBC/100 WBC | 0 | 0 |
Clinical Significance
CBC – The quantitative and qualitative analysis of the cellular elements of blood will identify imbalance between cell production, cell release, cell survival, or cell loss. This information increases the accuracy and specificity of diagnosis based on pathogenesis and is also used to monitor the effectiveness of therapy.
Automated Differential – The Differential distribution of white blood cells will, when correlated with absolute white cell count, identify imbalances between cell production, cell release, cell survival and/or cell loss. This information increases the accuracy and specificity of diagnosis based on pathogenesis and is also used to monitor the effectiveness of therapy.
Platelet Count – Platelets must be present in adequate numbers and have proper function to aid in hemostasis. A normal bleeding time is dependent on adequate platelet number and function.
Automated Differential – The Differential distribution of white blood cells will, when correlated with absolute white cell count, identify imbalances between cell production, cell release, cell survival and/or cell loss. This information increases the accuracy and specificity of diagnosis based on pathogenesis and is also used to monitor the effectiveness of therapy.
Platelet Count – Platelets must be present in adequate numbers and have proper function to aid in hemostasis. A normal bleeding time is dependent on adequate platelet number and function.
