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Reticulocyte Count, Percent, Automated
Test CodeRETP
Alias/See Also
Retic count, Retic percent, Retic %
Preferred Specimen
Adult: 5-7mL whole blood, based on tube size (1.0mL minimum)
Pediatric: 3mL whole blood (0.5mL minimum)
Neonate: 0.5mL whole blood in microtainer
Pediatric: 3mL whole blood (0.5mL minimum)
Neonate: 0.5mL whole blood in microtainer
Transport Container
EDTA (lavender/purple top)
Specimen Stability
Room temperature: 48 hours, Refrigerated: 2 days, Frozen: unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Clotted or grossly hemolyzed specimens are unacceptable.
Limitations
The following will interfere with results: Extreme temperatures, other erythrocyte inclusions that stain by new methylene blue dye, some hemoglobinopathies (SS, SC), and specimens older than 72 hrs.
Reference Range
Age | Male Female |
Adult (>19 years): | 0.5-1.8 % 0.5-1.7% |
Pediatric: | Male & Female |
0–13 days | 1.0 - 7.5 |
2 weeks-12 years | 0.6 - 2.7 |
13-18 years | 0.6 - 2.7 |
Clinical Significance
The enumeration of reticulocytes provides an effective means of determining red cell production and regeneration. Elevation is seen in patients with hemolytic anemia, hemorrhage (acute and chronic), treatment of iron-deficiency anemia and megaloblastic anemias and uremia. Decreased counts may be seen in aplastic anemia, aplastic crisis of hemolytic anemias and ineffective erythropoiesis as seen in thalassemia, pernicious anemia and sideroblastic anemia.