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Rotavirus Antigen
Test CodeROTAV
Includes
Source, Rotavirus
Preferred Specimen
1.0 mL Stool (50 uL minimum)
Instructions
Specimens should be collected after onset of symptoms.
Deliver to the laboratory promptly.
Deliver to the laboratory promptly.
Transport Container
Clean specimen container (or culturette if obtained from a diaper)
Specimen Stability
Room temperature: submit as soon as possible, Refrigerated: 72 hours, Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Meconium stools are unacceptable.
Methodology
Immunoassay
Limitations
Specimens containing high levels of blood may fail to flow in the test device, resulting in an invalid test result. Testing of an additional specimen is recommended under such circumstances. This test does not define the presence of rotavirus-associated gastroenteritis, but only demonstrates the presence of the antigen in stools. A positive result does not preclude the presence of other infective organisms. Intestinal infection with bacterial pathogens may be present simultaneously with Rotavirus infection. Therefore, perform bacterial testing in parallel with the Rotavirus assay.
Reference Range
Negative
Clinical Significance
This test is a rapid in vitro qualitative procedure for the detection of rotavirus antigen in human stool. The test can be used to aid in the diagnosis of rotavirus-associated gastroenteritis.