MPL Mutation Analysis

Test Code

MPL Mut-QNI

Quest Code

92476

CPT Codes
81339 (NOT COVERED by CMS DEX Recommended 81479 ZB11T)

Preferred Specimen

4 mL whole blood or 3 mL bone marrow aspirate collected in an EDTA (lavender-top) tube

Minimum Volume

3 mL whole blood • 1 mL bone marrow aspirate

Other Acceptable Specimens

Whole blood or bone marrow aspirate collected in: Sodium heparin (green-top) tube • Fixed-cell pellet collected in: Plastic leak-proof container • Extracted DNA from CLIA-certified laboratory collected in: Sterile leak-proof container

Instructions

Cell pellets: Send fixed samples within 7 days of fixation date in a plastic, leak-proof tube/container. Indicate type of fixative used along with patient name, specimen source, date collected, and date harvested/fixed. Cell pellets submitted in Carnoy's or other alcohol-based fixatives are acceptable. Do not reject specimens, send to laboratory for screening.

Transport Temperature

Refrigerated

Specimen Stability

Room temperature: 7 days
Refrigerated: 7 days
Frozen: Unacceptable

Extracted DNA
Room temperature:7 days
Refrigerated: 14 days
Frozen: 1 year

Methodology
Next-Generation Sequencing

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Daily; Report available: 5-7 days

Reference Range

See Laboratory Report

Clinical Significance

This advanced DNA-based, next-generation sequencing (NGS) assay analyses leukocytes from blood or bone marrow aspirate for mutations in exon 10 of the thrombopoietin receptor (MPL), including condons 505 and 515. Mutations at these sites in MPL are associated with myeloproliferative neoplasms (MPN), particularly essential thrombocythemia (ET) and primary myelofibrosis (PMF).

Performing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.