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Cytomegalovirus DNA, Qualitative, Real-Time PCR
Test CodeCMVPCRQL
Quest Code
10601
Alias/See Also
10601
CPT Codes
87496
Preferred Specimen
SST 1.0 mL Serum OR 1 mL whole blood collected in an EDTA (lavender-top) or ACD (yellow-top) tube Plasma collected in an EDTA (lavender-top), ACD B (yellow-top), or PPT Potassium EDTA (white-top) tube CSF, Bronchoalveolar lavage, Eye fluids, Amniotic fluid, Amniotic fluid supernatant collected in a sterile leak-proof container OR random urine collected in a sterile screw cap container
Minimum Volume
3 cubic mm tissue • 0.25 mL vitreous fluid • 0.5 mL all others
Other Acceptable Specimens
3 cubic mm fresh (unfixed) tissue collected in a sterile plastic leak-proof container
Instructions
Whole blood: Collect whole blood in sterile tubes containing EDTA anticoagulant. Store refrigerated. Do not freeze whole blood.
Plasma: Collect blood in sterile tubes containing EDTA anticoagulant or in plasma preparation tubes (PPT). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-cap tubes and store refrigerated or frozen. If blood is collected in a PPT, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT to aliquot tubes.
Serum: Collect in a red-top tube (no gel) or serum separator tube (SST) and store refrigerated or frozen.
CSF, amniotic fluid, urine, BAL, vitreous (eye) fluid: Collect in a sterile, plastic, leakproof container and store refrigerated or frozen.
Tissue: Fresh (unfixed) tissue submitted in a sterile, plastic, leakproof container and store refrigerated or frozen.
Plasma: Collect blood in sterile tubes containing EDTA anticoagulant or in plasma preparation tubes (PPT). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-cap tubes and store refrigerated or frozen. If blood is collected in a PPT, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT to aliquot tubes.
Serum: Collect in a red-top tube (no gel) or serum separator tube (SST) and store refrigerated or frozen.
CSF, amniotic fluid, urine, BAL, vitreous (eye) fluid: Collect in a sterile, plastic, leakproof container and store refrigerated or frozen.
Tissue: Fresh (unfixed) tissue submitted in a sterile, plastic, leakproof container and store refrigerated or frozen.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Whole blood
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
All other sample types
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
All other sample types
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Heparinized specimens • Unspun PPT tube • Paraffin embedded (FFPE) tissue
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 2-3 days
Reference Range
Not detected
Clinical Significance
In patients who are immunocompromised, CMV may cause disseminated, severe disease. CMV is also the most common cause of congenital viral infection in humans. Quantitative PCR methods may be useful in monitoring CMV replication in immunosuppressed patients or in determining the viral load of CMV in amniotic fluid.
Performing Laboratory
| Quest Diagnostics Nichols Institute |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |
