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Semen Post-Vasectomy check
Test CodeSEMENPV
Preferred Specimen
Specimen is produced by masturbation.
- The entire ejaculate must be collected in a clean screw-cap container provided by the laboratory or physician’s office
- If the specimen is collected outside of the lab it is important that it be delivered within 30 minutes of collection
Patient Preparation
Patient should be abstinent for 2-7 days prior to collection. Provide patient with copy of “Instructions for collection of semen for analysis”.
Minimum Volume
A minimum volume of 1mL of semen is required.
Specimen Stability
Storage Temperature: The specimen should be protected from extreme temps (<20°C or >40°C) and brought to the lab within 30 minutes of collection. Ask the patient when the specimen was collected and document in the computer.
Stability after Collection: 24 hours
Sample Retention: 24 hours
Stability after Collection: 24 hours
Sample Retention: 24 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimen is received >24 hours after collection.
Specimen is collected in a condom, or with the use of lubricants or other contaminants.
Specimen is collected in a condom, or with the use of lubricants or other contaminants.
Limitations
Method Limitations • Sperm may be missed if liquification is not complete or sample is not well-mixed. • This procedure is limited to post-vasectomy only. If semen analysis for fertility workup is ordered (to include sperm count, motility, etc), refer to closest reference lab that performs full semen analysis.
Reference Range
Reference Intervals
Sperm: Absent
Motility: Non-motile
Sperm: Absent
Motility: Non-motile
Clinical Significance
Post vasectomy semen checks are performed monthly to determine when sterilization is complete. A patient is considered sterile when two sperm checks, performed on samples collected a month apart, are negative for the presence of sperm cells.
