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BK Virus Quantitative PCR (BUMCT) [Blood]
MessagePerformed at BUMCT
No more than one collection within 72 hours will be accepted.
No more than one collection within 72 hours will be accepted.
Test Code
BKPCR-
Alias/See Also
Plasma, BK virus, Quantitative, Viral Load, PCR, qPCR, Tucson
Preferred Specimen
3-6mL Lavender (EDTA Plasma)
Minimum Volume
1.5mL (Pediatric).
Transport Temperature
In-house (BUMCT ONLY):
• EDTA specimen may be sent at ambient temperature, plasma must be separated within 6 hours of collection.
Other sites:
• Centrifuge EDTA specimen at 1200xg for 20 mins and transfer no more than 1.8mL of plasma. Note: the absolute minimum volume acceptable is 0.5mL of plasma. After centrifugation, plasma must be sent FROZEN to BUMCT.
Methodology
Qiagen Rotorgene-Q
Setup Schedule
Tuesday, Wednesday, and Friday (Cutoff: 8:30am)
Report Available
1-7 Days
Clinical Significance
A real-time PCR assay of extracted nucleic acids for the in vitro quantitative detection of BK virus in plasma. Limit of Detection: 100 copies/mL Detected, but below limit of quantitation: 100-315 copies/mL Limit of Quantitation: 316-7.48x10e8 cop/mL.
This test was developed, and its performance characteristics determined by Banner Health/Laboratory Sciences of Arizona. It has not been cleared or approved by the U.S. Food and drug Administration (FDA). The FDA has determined that such clearance or approval is not necessary since the laboratory is approved under CLIA for high complexity testing.
This test was developed, and its performance characteristics determined by Banner Health/Laboratory Sciences of Arizona. It has not been cleared or approved by the U.S. Food and drug Administration (FDA). The FDA has determined that such clearance or approval is not necessary since the laboratory is approved under CLIA for high complexity testing.
