Vitamin D, 25-Hydroxy, Fractionated LC/MS/MS

Message
For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.


Test Code
VD25FR


Alias/See Also
75672;
D2 & D3;
D2 & D3 Fractionated;
Vit D 25 D2 & D3;
Vit D 25 OH D2 & D3;
Vitamin D 25 Hydroxy D2 & D3;
Vitamin D 25 OH D2 & D3;
25 Hydroxy Vit D D2 & D3;
25 OH Vit D D2 & D3;
25 OH Vitamin D D2 & D3


CPT Codes
82306

Preferred Specimen
Serum


Minimum Volume
2 mL


Instructions
Avoid hemolysis. Allow to clot in an upright position for 30 minuties. Centrifuge for at least 10 minutes. Keep tightly stoppered.


Transport Container
original tube


Transport Temperature
Room Temperature or Refrigerated


Specimen Stability
Room Temperature = 3 weeks
Refrigerated = 3 weeks
Frozen = 3 weeks


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis, Lipemia, incorrect specimen type, insufficient sample volume, improper storage conditions, exceeds specimen stability guidelines, contaminated sample.


Methodology
LC/MS/MS

FDA Status
This test was developed and its performance characteristics determined by CompuNet Clinical Labs. The laboratory is regulated under CLIA as qualified to perform high-complexity testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.

Setup Schedule
Tuesday and Friday
TX


Report Available
5 days


Clinical Significance
Both D2 and D3 portions of Vitamin D are measured in this assay, usually utilized to monitor intensive thereapy. This assay employs liquid chromatography tandem mass spectrometry.


Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, OH 45439



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.