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Legionella Pneumophila Antigen, DFA
MessageFor Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.
Test Code
LEGNAG
Alias/See Also
34475
CPT Codes
87278
Preferred Specimen
1 gram fresh (unfixed)lung or other respiratory tissue submitted in a sterile leak proof container or 3 mL bronchial or transtracheal wash/lavbage/aspirate or sputum or pleural fluid submitted in a sterile leak-proof container
Minimum Volume
1 gram lung tissue or other respiratory tissue • 2 mL respiratory fluids
Instructions
Lung Tissue: Place into sterile container with a small amount of sterile, non-bacteriostatic, distilled water. Do not add saline beccause it may be inhibitory.
Sputum: Instruct patient to gargel with water and cough deeply to collect deep respiratory specimen in sterile container.
Sputum: Instruct patient to gargel with water and cough deeply to collect deep respiratory specimen in sterile container.
Transport Container
Sterile leak-proof container
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: Unacceptable
Refrigerated: 7 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Slides: serum; urine; expired transport device; environmental sources; specimens received in a viral transport media
Methodology
Direct Immunofluorescence Assay (DFA)
Setup Schedule
Set up: Mon-Sat; Report available: Next day
Clinical Significance
Bacteria in the genus Legionella primarily cause respiratory illness, i.e., either Legionnaires' Disease, a systemic illness manifested primarily by pneumonia, or Pontiac Fever, a non-pneumonic, influenza-like illness. Legionellae may also cause a variety of other illnesses.
Performing Laboratory
| Quest Diagnostics Nichols Institute |
| 14225 Newbrook Drive |
| Chantilly, VA 20153 |
