Adenovirus DNA, Qualitative Real-Time PCR

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Test Code

ADENQL

Alias/See Also

16046

CPT Codes
87798

Preferred Specimen

Throat or nasopharyngeal swab submitted in 1 mL (0.6 mL minimum) M4 media, VCM (green-cap), or equivalent (UTM) tube

Minimum Volume

3 cubic mm tissue • 0.6 mL all other specimen types

Other Acceptable Specimens

Serum, Whole blood collected in: EDTA (lavender-top), Plasma collected in: EDTA (lavender-top) or PPT (white-tope), CSF, bronchial lavage/wash, sputum, nasopharyngeal lavage/wash, tracheal lavage/wash, urine, 3 cubic mm tissue collected in a sterile plastic leak-proof container, Eye swab (conjunctival) in a M4 VTM tube or equivalent.

Transport Temperature

Tissue and Sputum: Frozen
All other specimens: Refrigerated

Specimen Stability

Whole blood & Urine
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable
Sputum:
Room Temperauture: Unacceptable
Refrigerated: Unacceptable
Frozen; 30 days
All other specimens
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Samples collected in sodium or lithium heparin; calcium alginate swabs.

Methodology
Real-Time Polymerase Chain Reaction

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Daily; Report available: 1 day

Clinical Significance

This test is designed to detect multiple strains of adenovirus, including adenovirus 40 and 41, but it does not differentiate among strains. Organisms may be detected by PCR prior to diagnosis by immunological methods. PCR provides more rapid results than other methods and is the preferred method for the detection of acute adenovirus infection.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.