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CMV QUALITATIVE PCR
Test CodeCMVQLP
CPT Codes
87496
Preferred Specimen
Urine collected from bags, containers, and/or catheters
Saliva flocked swab specimens transported in VTM
Saliva flocked swab specimens transported in VTM
Minimum Volume
0.2 mL Urine and 3 mL VTM
Instructions
To collect a saliva sample from a baby, gently insert the swab into the mouth and collect pooling saliva at the corners of the mouth or under the tongue. Rotate the swab for 10-15 seconds and move it to the opposite side of the baby’s mouth to repeat the process.
Transport Container
Urine: Sterile cup
Saliva: VTM
Saliva: VTM
Transport Temperature
Urine: Frozen
Saliva in VTM: Refrigerated
Saliva in VTM: Refrigerated
Specimen Stability
Urine
Room Temperature: 2 hours
Refrigerated: 8 hours
Frozen: 3 days at -20C, at least 3 days at -70C
Saliva
Room Temperature: 2 days
Refrigerated: 7 days
Frozen: 14 days at -20C, at least 14 days at -70C
Room Temperature: 2 hours
Refrigerated: 8 hours
Frozen: 3 days at -20C, at least 3 days at -70C
Saliva
Room Temperature: 2 days
Refrigerated: 7 days
Frozen: 14 days at -20C, at least 14 days at -70C
Methodology
Real Time Polymerase Chain Reaction (PCR) for the amplification of a well conserved region of CMV UL83 Gene.
Setup Schedule
Monday – Sunday (MD)
Report Available
1 day
Limitations
Positive results from saliva are presumptive and should be confirmed with urine
Clinical Significance
This CMV assay is a real-time PCR test that enables the direct amplification and detection of CMV DNA from either saliva swab or urine specimens.
