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Culture, Stool
Test CodeSTLC
Includes
Salmonella, Shigella, campylobacter, and E. coli O157:H7
Preferred Specimen
Stool submitted in Cary-Blair stool culture transport medium
Minimum Volume
1 gram or 1 mL
Other Acceptable Specimens
Raw stool if received in lab iwthin 2 hours of collection.
Instructions
Stool: Collect stool in a clean, dry container and then transfer to an enteric transport vial containing modified Cary-Blair medium.
Swab: Place rectal swab in a modified Cary-Blair transport medium or Amies transport medium.
Swab: Place rectal swab in a modified Cary-Blair transport medium or Amies transport medium.
Transport Container
Modified Cary-Blair transport medium or
Amies liquid elution swab (ESwab®) or
Amies liquid or gel transport swab or equivalent
Amies liquid elution swab (ESwab®) or
Amies liquid or gel transport swab or equivalent
Transport Temperature
Ambient
Specimen Stability
Cary-blair transport medium:
Ambient: 3 days
Ambient: 3 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unpreserved, >2 hours old, Frozen specimens in Cary-Blair transport medium, Expired transport medium, Specimen in diaper or parasitology transport vial
Methodology
Bacterial Culture • Aerobic isolation and identification procedures
Setup Schedule
24/7
Report Available
3 days
Limitations
Diarrhea may be caused by a wide variety of organisms and other factors.
Reference Range
Not isolated
Clinical Significance
Salmonella and shigella represent two of the most common bacterial causes of diarrhea. If other common causes of diarrhea are suspected, they must be ordered individually. For many patients, the cause of gastrointestinal infection is not identified.
