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LYNCH SYNDROME, MSH2 SEQUENCING AND DELETION/DUPLICATION(INCLUDING EPCAM)
MessageSENDOUT/QUEST DIRECT SHIP-SAN JUAN
Test Code
MSH2 SEQ
Alias/See Also
LYNCH SYNDROME, Hereditary Nonpolyposis Colon Cancer (HNPCC),HNPCC Mismatch Repair Gene Mutation
Preferred Specimen
EDTA(2) LAV TOP TUBES-WHOLE BLOOD
Minimum Volume
2 mL whole blood
Collection Instructions
Saliva: 2 mL saliva collected in the Oragene-Dx collection kit up to the “fill to" line (OGD-500/OGD-600, OGD-510/OGD-610). Do NOT eat, drink, smoke or chew gum for 30 minutes before collection. Do NOT remove the plastic film from the funnel lid. Once sample is collected, tighten the cap on the tube. Please follow included instructions for use.
For amniotic fluid, chorionic villus (CVS), and skin fibroblasts: Call 1-866-GENEINFO (1-866-436-3463) prior to collecting and ordering.
Collection Instructions
Saliva: 2 mL saliva collected in the Oragene-Dx collection kit up to the “fill to" line (OGD-500/OGD-600, OGD-510/OGD-610). Do NOT eat, drink, smoke or chew gum for 30 minutes before collection. Do NOT remove the plastic film from the funnel lid. Once sample is collected, tighten the cap on the tube. Please follow included instructions for use.
For amniotic fluid, chorionic villus (CVS), and skin fibroblasts: Call 1-866-GENEINFO (1-866-436-3463) prior to collecting and ordering.
Other Acceptable Specimens
Saliva collected in the Oragene-Dx collection kit or
15 mL amniotic fluid collected in sterile container or
20 mg chorionic villus (CVS) tissue with 2-3 mL of sterile saline or tissue culture medium collected in sterile container or
Cultured cells in 2 T-25 flasks with 80-100% confluent growth or
Skin fibroblasts collected in sterile plastic container
For amniotic fluid, chorionic villus (CVS), and skin fibroblasts, call 1-866-GENEINFO (1-866-436-3463) prior to collecting and ordering.
15 mL amniotic fluid collected in sterile container or
20 mg chorionic villus (CVS) tissue with 2-3 mL of sterile saline or tissue culture medium collected in sterile container or
Cultured cells in 2 T-25 flasks with 80-100% confluent growth or
Skin fibroblasts collected in sterile plastic container
For amniotic fluid, chorionic villus (CVS), and skin fibroblasts, call 1-866-GENEINFO (1-866-436-3463) prior to collecting and ordering.
Instructions
TEST IS SET UP ON TUES, THURS, SAT ONLY. COLLECTION OF SAMPLES MIDWEEK MUST BE SHIPPED DIRECTLY.
TO SAN JUAN.****MUST ALSO INCLUDE REPORT OF RESULTS FOR FAMILY MEMBER WITH KNOW MUTATION*******
TO SAN JUAN.****MUST ALSO INCLUDE REPORT OF RESULTS FOR FAMILY MEMBER WITH KNOW MUTATION*******
Transport Container
ORIGINAL TUBES-DIRECT SHIP
Transport Temperature
ROOM TEMP
Specimen Stability
Whole blood
Room temperature: 14 days
Refrigerated: 14 days
Frozen: Unacceptable
Saliva
Room temperature: 14 days
Refrigerated: 14 days
Frozen: 14 days
Amniotic fluid (AF), chorionic villus (CVS), skin fibroblasts
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Room temperature: 14 days
Refrigerated: 14 days
Frozen: Unacceptable
Saliva
Room temperature: 14 days
Refrigerated: 14 days
Frozen: 14 days
Amniotic fluid (AF), chorionic villus (CVS), skin fibroblasts
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
CLOTTER WHOLE BLOOD
Methodology
DNA Bait Capture • Long Range Polymerase Chain Reaction • Next Generation Sequencing • Microarray
Setup Schedule
Tues, Thurs, Sat; Report available: 14-21 days from completed pre-authorization
Performing Laboratory
Quest Diagnostics Nichols Institute San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92675
Additional Information
LYNCH SYNDROME, MSH2 SEQUENCING AND DELETION/DUPLICATION(INCLUDING EPCAM)
