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VAGINOSIS PANEL
MessageMICROBIOLOGY
Test Code
VAGINOSIS PANEL
Alias/See Also
TRICHOMONAS, GARDNERELLA, CANDIDA, VAGINITIS, VAGINOSIS, DNA PROBE VAGINITIS PANEL
Includes
TRICHOMONAS PROBE, GARDNERELLA PROBE, CANDIDA PROBE, INTERNAL CONTROL
Preferred Specimen
AFFRIM KIT
Minimum Volume
10mLs
Instructions
A. Preparation of Transport System and Collection of Vaginal Sample. Sample Collection is a critical step. Personnel collecting vaginal fluid specimens for transport with this system should be well trained on adequate
sample collection technique. All samples for use with the Affirm VPIII Microbial Identification Test must be collected using the materials providedwith this system. If not received per collection instructions below, or received >72 hours after collection, the specimen will be rejected.
1. Open the seal on outer plastic pouch of Affirm VPIII Ambient Temperature Transport System and remove all components [each plastic pouch contains enoughmaterial for the collection and transport of one vaginal specimen].
2. Tear open the foil pouch and remove the ATTS Reagent Dropper.
3. Break ampule in ATTS Reagent Dropper by firmly squeezing vial with finger and thumb. CAUTION: Break ampule close to its center one time only. Do not manipulate dropper any further, as the plastic may puncture and injury may occur. 4. Dispense reagent from ATTS Reagent Dropper into Sample Collection Tube.
5. Peel wrapper to expose patient swab. Remove swab. Discard wrapper.
6. Collect patient specimen. Properly identify the patient according to Center Memorandum entitled "Specimen Labeling and Specimen Acceptance Criteria," by using 2 identifiers. - Place the patient in position for a
pelvic examination. Insert an UNLUBRICATED speculum (WITHOUT JELLY OR WATER) into the vagina to permit visualization of the posterior vaginal fornix. - Using the sterile swab, obtain a sample from the posterior vaginal fornix. Twist or roll the swab against the vaginal wall two or three times, ensuring the entire circumference of the swab has touched the vaginal wall. Swab the lateral vaginal wall while removing the swab.
7. Immediately place the patient swab in the Sample Collection Tube containing the ATTS Reagent.
8. Break swab shaft at pre-scored line just above the top of the tube. Discard remaining shaft into a biohazard waste container.
9. Place the Sample Collection Cap over the exposed end of the swab and firmly press the cap onto the Sample Collection Tube. The cap will 'snap' onto
the tube when it is properly seated.
10. Label the Sample Collection Tube with patient identification information per facility policy. Include date and time that sample was taken.
B. Transport and Storage of Vaginal Sample
Send the capped Sample Collection Tube to the lab by sealing it in the emptied outer plastic pouch of the Affirm VPIII Ambient Temperature TransportSystem. Include the empty ATTS Reagent Dropper for proper disposal.
LAB PROCESSING INSTRUCTIONS : All samples for use with the Affirm VPIII Microbial Identification Test must be collected using the materials provided
with this system. If not received per collection instructions below, or received >72 hours after collection, the specimen will be rejected. Bring to the Microbiology Department and place in the specimen refrigerator.
sample collection technique. All samples for use with the Affirm VPIII Microbial Identification Test must be collected using the materials providedwith this system. If not received per collection instructions below, or received >72 hours after collection, the specimen will be rejected.
1. Open the seal on outer plastic pouch of Affirm VPIII Ambient Temperature Transport System and remove all components [each plastic pouch contains enoughmaterial for the collection and transport of one vaginal specimen].
2. Tear open the foil pouch and remove the ATTS Reagent Dropper.
3. Break ampule in ATTS Reagent Dropper by firmly squeezing vial with finger and thumb. CAUTION: Break ampule close to its center one time only. Do not manipulate dropper any further, as the plastic may puncture and injury may occur. 4. Dispense reagent from ATTS Reagent Dropper into Sample Collection Tube.
5. Peel wrapper to expose patient swab. Remove swab. Discard wrapper.
6. Collect patient specimen. Properly identify the patient according to Center Memorandum entitled "Specimen Labeling and Specimen Acceptance Criteria," by using 2 identifiers. - Place the patient in position for a
pelvic examination. Insert an UNLUBRICATED speculum (WITHOUT JELLY OR WATER) into the vagina to permit visualization of the posterior vaginal fornix. - Using the sterile swab, obtain a sample from the posterior vaginal fornix. Twist or roll the swab against the vaginal wall two or three times, ensuring the entire circumference of the swab has touched the vaginal wall. Swab the lateral vaginal wall while removing the swab.
7. Immediately place the patient swab in the Sample Collection Tube containing the ATTS Reagent.
8. Break swab shaft at pre-scored line just above the top of the tube. Discard remaining shaft into a biohazard waste container.
9. Place the Sample Collection Cap over the exposed end of the swab and firmly press the cap onto the Sample Collection Tube. The cap will 'snap' onto
the tube when it is properly seated.
10. Label the Sample Collection Tube with patient identification information per facility policy. Include date and time that sample was taken.
B. Transport and Storage of Vaginal Sample
Send the capped Sample Collection Tube to the lab by sealing it in the emptied outer plastic pouch of the Affirm VPIII Ambient Temperature TransportSystem. Include the empty ATTS Reagent Dropper for proper disposal.
LAB PROCESSING INSTRUCTIONS : All samples for use with the Affirm VPIII Microbial Identification Test must be collected using the materials provided
with this system. If not received per collection instructions below, or received >72 hours after collection, the specimen will be rejected. Bring to the Microbiology Department and place in the specimen refrigerator.
Transport Container
AFFRIM KIT
Transport Temperature
ROOM TEMP
Setup Schedule
DAILY
Performing Laboratory
WHITE RIVER JUNCTION VT VA- MICROBIOLOGY 802-295-9363 EXT: 5514
