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HELICOBACTER PYLORI STOOL ANTIGEN
MessageSENDOUT/BOSTON
Test Code
HELI-STOOL Ag
Alias/See Also
H. PYLORI STOOL
Preferred Specimen
Solid or formed, semisolid and liquid stool samples are acceptable.
Instructions
Antimicrobials, proton pump inhibitors and bismuth preparations are known to suppress H. pylori, and ingestion of these prior to H. pylori testing (culture, histology, rapid urease, UBT, antigen) may cause false-negative results. If a patient has ingested these compounds within two weeks prior to performing the ImmunoCard STAT! HpSA test, a false-negative result may occur. In such cases, the test should be repeated on a new specimen obtained two weeks after discontinuing treatment. A positive result for a patient ingesting these compounds within two weeks prior to performing the ImmunoCard STAT! HpSA test, should be considered accurate.
Transport Container
STERILE CONTAINER
Transport Temperature
COLLECT STOOL IN A STERILE CONTAINER PROVIDED BY PHYSICIAN OFFICE OR LAB. MAKE SURE FULL NAME, FULL SSN, DATE/TIME OF COLLECTION IS ON THE LABEL. SEND TO LAB
Specimen Stability
Refrigerated 2-8 C for 72 hours
Frozen -20 up to 14 days
The specimen should be transported in an airtight container and stored at 2-8 C until tested. The specimen should be tested as soon as possible, but may be held up to 72 hours at 2-8 C prior to testing. If testing cannot be performed within this time frame, specimens should be frozen immediately upon receipt and stored frozen (≤ -20 C) until tested. Specimens may be frozen and thawed twice.
Frozen -20 up to 14 days
The specimen should be transported in an airtight container and stored at 2-8 C until tested. The specimen should be tested as soon as possible, but may be held up to 72 hours at 2-8 C prior to testing. If testing cannot be performed within this time frame, specimens should be frozen immediately upon receipt and stored frozen (≤ -20 C) until tested. Specimens may be frozen and thawed twice.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
QNS
DO NOT USE STOOL IN TRANSPORT MEDIA, OR SWABS, OR MIXED WITH PRESERVATIVES.
DO NOT USE STOOL IN TRANSPORT MEDIA, OR SWABS, OR MIXED WITH PRESERVATIVES.
Methodology
Fluorescent Immunoassay
Limitations
Interfering substances:
The following substances were found to have no effect on results when present in stool at the concentrations indicated.
Tums® Antacid (5 mg/mL), Tagamet® (5 mg/mL), Prilosec® (5 mg/mL), Mylanta® Antacid (1:20), Pepto-Bismol® (1:20), Barium sulfate (5%), Whole Blood (50%), Leukocytes (50%), Mucin (3.4%), Stearic acid/palmitic acid (fecal fat) (4%), Hemoglobin (tarry stool) (12.5%)
The following substances were found to have no effect on results when present in stool at the concentrations indicated.
Tums® Antacid (5 mg/mL), Tagamet® (5 mg/mL), Prilosec® (5 mg/mL), Mylanta® Antacid (1:20), Pepto-Bismol® (1:20), Barium sulfate (5%), Whole Blood (50%), Leukocytes (50%), Mucin (3.4%), Stearic acid/palmitic acid (fecal fat) (4%), Hemoglobin (tarry stool) (12.5%)
Reference Range
NEG
Performing Laboratory
Testing performed in the WX Immunology Lab. Contact: Robert Boc 857-203-5941
Additional Information
H. PYLORI Ag STOOL
