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Pneumocystis jirovecii, Quantitative, Real-Time PCR
Test Code18834
Alias/See Also
LAB723
Preferred Specimen
1 mL bronchoalveolar lavage (BAL) or bronchial wash submitted in a sterile leak-proof container
Minimum Volume
0.5 mL
Transport Temperature
Refrigerated (cold-packs)
Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Refrigerated: 7 days
Frozen: 30 days
Methodology
Real-Time Polymerase Chain Reaction (PCR)
Setup Schedule
Set up: Mon-Sat; Report available: 2-3 days
Clinical Significance
This test is intended for HIV-positive and immunocompromised individuals with clinical presentation and/or radiographic findings consistent with P. jirovecii infection. This test can be used to aid in the diagnosis of P. jirovecii pneumonia (PCP) enabling the initiation of specific therapy. The PCR test has higher sensitivity and specificity when compared to alternative diagnostic methods such as staining (GMS, Calcofluor white, DFA), (1,3) β-D-glucan test or imaging studies. Due to the analytical sensitivity of this assay, the test can be positive in colonized individuals without clinical evidence of PCP. The reportable range of this test is 250 to 125,000,000 copies/mL (2.40 to 8.10 Log copies/mL). The results of this test should be interpreted in the context of pertinent clinical findings and history.
Reference
1. Bateman M, et al. Medical Mycology. 2020;58 :1015-1028.
Reference
1. Bateman M, et al. Medical Mycology. 2020;58 :1015-1028.
